碱蓬根系嗜盐耐盐真菌的分离与鉴定

Isolation and identification of halophilic and halotolerant fungi from roots of Suaeda plants

为分析耐盐植物碱蓬根系真菌的组成和耐盐程度,采用常规植物组织真菌分离方法对盐碱地中生长健康的碱蓬根系进行真菌分离,将分离得到的真菌在不同Na Cl浓度培养基上进行培养,检测菌株的嗜盐耐盐性,并对分离得到的嗜盐耐盐真菌进行形态学鉴定和分子生物学鉴定。结果显示:分离得到耐盐真菌40株,分别属于镰刀菌属(Fusarium sp.)、链格孢属(Alternaria sp.)、曲霉属(Aspergillus sp.)、腐霉属(Pythium sp.)等,其中,棘孢曲霉(Aspergillus aculeatus)和尖孢镰刀菌(Fusarium oxysporum)是没有被报道过的耐盐真菌;分离得到嗜盐真菌3株,都属于聚多曲霉(Aspergillus sydowii)。结果表明,耐盐植物碱蓬中嗜盐耐盐真菌多样性丰富。

In the present study,the traditional tissue-isolation method was used to isolate fungi from suaeda roots collected from Inner Mongolian for investigation of fungi composition structure and their salinity tolerance.Salinity tolerance of fungi were evaluated by fungal incubating method on PDA media with different concentrations of NaCl.The halophilic and halotolerant fungi were screened out and identified via both morphological characters and ITS rDNA sequencing.A total of 40 halotolerant fungi strains were isolated in this study,and were preliminary classified as Fusarium sp.,Alternaria sp.,Aspergillus sp.,Pythium sp.,etc.Among these fungi,Aspergillus aculeatus,Fusarium oxysporum were newly recorded halotolerant fungi.Three halophilic fungi were isolated and all were classified as Aspergillus sydowii.Results of the present research indicated that thediversity of salinity-tolerance fungi was high in the halotolerant plant rhizosphere.