温州市非结核分枝杆菌菌种的地域分布特点

Geographical distribution characteristics of nontuberculous Mycobacterium species in Wenzhou

【目的】了解温州市11个区县呼吸道非结核分枝杆菌(NTM)的菌种分布情况。【方法】对2014年1月至2017年5月分离鉴定的NTM菌株,利用16S rRNA和hsp65基因测序或基因芯片进一步鉴定至种。【结果】菌种鉴定为NTM 293株(已剔除重复菌株),NTM种类达15种,以鸟分枝杆菌复合群(MAC)和脓肿分枝杆菌占绝对优势(89.4%,262/293),其他菌种仅占10.6%(31/293)。分离NTM菌株数居前6位的区县依次为鹿城(28.7%,84/293)、瓯海(13.7%,40/293)、乐清(12.6%,37/293)、永嘉(11.6%,34/293)、苍南(10.6%,31/293)和瑞安(9.6%,28/293)。鹿城、龙湾、瓯海、瑞安、永嘉和平阳等6个区县的前3位菌种均依次为胞内分枝杆菌、脓肿分枝杆菌和鸟分枝杆菌,胞内分枝杆菌是除苍南外各区县最常见的菌种。【结论】温州市各区县的NTM菌种主要为胞内分枝杆菌、脓肿分枝杆菌和鸟分枝杆菌,地域分布存在一定差异。

[Objective]To study the distribution of respiratory nontuberculosis Mycobacteria(NTM)in 11 districts and counties of Wenzhou.[Methods]All identified NTM strains isolated were further identified by 16S rRNA and hsp65 gene sequencing or gene chip during January 2014 to May 2017.[Results]A total of 293 strains were identified as NTM(rejection of duplicate strains),of which there were 15 species.Mycobacterium avium complex(MAC)and Mycobacterium abscess had the absolute superiority(89.4%,262/293),and the other strains accounted for only 10.6%(31/293).The top six districts and counties where NTM strains were separated from were Lucheng(28.7%,84/293),Ouhai(13.7%,40/293),Leqing(12.6%,37/293),Yongjia(11.6%,34/293),Cangnan(10.6%,31/293)and Ruian(9.6%,28/293)accounting for the majority(86.7%,254/293).While the other five districts and counties accounted for only 13.3%(39/293).The distribution of Mycobacterium intracellulare was the most in Lucheng,Longwan,Ouhai,Ruian,Yongjia and Pingyang,followed by Mycobacterium abscess and Mycobacterium avium,and Mycobacterium intracellulare was most frequently isolated in all districts and counties except Cangnan.[Conclusion]The NTM species in Wenzhou are mainly composed of Mycobacterium intracellulare,Mycobacterium abscess and Mycobacterium avium,But there are some geographical distribution differences existing in them.