丁酸通过下调HMGB1抑制高糖诱导下结肠上皮细胞NCM460的异常增殖

Butyrate suppresses abnormal proliferation of colonic epithelial NCM460 cells induced by high glucose by down-regulating HMGB1

目的观察丁酸对高葡萄糖诱导下结肠上皮细胞NCM460增殖的影响,探讨高迁移率族蛋白1(HMGB1)/晚期糖基化终产物受体(RAGE)通路在其中的作用。方法用33 mmol/L D-葡萄糖诱导正常人结肠细胞系NCM460,并予4 mmol/L丁酸干预,通过实时荧光定量PCR、蛋白免疫印迹法、细胞免疫荧光及ELISA检测细胞HMGB1和RAGE的表达情况。测定增殖细胞核抗原(PCNA)表达量和细胞增殖的情况。过表达及下调NCM460的HMGB1,观察HMGB1对细胞表达RAGE及增殖的调控作用。结果高糖诱导下,NCM460细胞增殖能力增高,HMGB1和RAGE的表达升高,细胞上清中HMGB1的含量亦增加(P均<0.001)。予丁酸处理,NCM460细胞异常增殖受抑制且HMGB1和RAGE的表达下降(P均<0.001)。过表达HMGB1可上调RAGE的表达并逆转丁酸对异常增殖的抑制作用,而下调HMGB1表达可抑制RAGE的表达,抑制异常增殖(P均<0.05)。结论高糖诱导下的NCM460存在增殖异常,HMGB1和RAGE表达增加。丁酸通过下调HMGB1/RAGE通路,抑制高糖诱导下NCM460的异常增殖。

Objective To observe the effect of butyrate on the proliferation of colonic epithelial NCM460 cells induced by high glucose and to explore the role of high-mobility group box 1 protein(HMGB1)/the receptor for advanced glycation end products(RAGE)pathway during this process.Methods The normal human colonic cell lines NCM460 were induced by D-glucose at a dosage of 33 mmol/L and treated with butyrate at a dose of 4 mmol/L.The expression levels of HMGB1 and RAGE in the treated NCM460 cells were quantitatively measured by real-time fluorescent quantitative PCR(RT-qPCR),western blot analysis,immunofluorescent staining.The expression level of proliferating cell nuclear antigen(PCNA)was measured and the cell proliferation was investigated.The level of HMGB1 was over-expressed and down-regulated in NCM460 cells.The effect of HMGB1 upon the expression level of RAGE and the proliferation of NCM460 cells was assessed.Results Under high-glucose induction,the proliferation of NCM460 cells was accelerated,the expression levels of HMGB1 and RAGE were significantly up-regulated and the content of HMGB1 was considerably elevated in the NCM460 cells(all P<0.001).After butyrate treatment,the abnormal proliferation of NCM460 cells was effective suppressed and the expression levels of HMGB1 and RAGE were significantly down-regulated(all P<0.001).Over-expression of HMGB1 could up-regulate the expression of RAGE and reverse the inhibitory effect of butyrate on abnormal cellular proliferation,whereas down-regulation of HMGB1 expression could inhibit the expression of RAGE and suppress abnormal cellular proliferation(all P<0.05).Conclusions Under high-glucose induction,abnormal proliferation of NCM460 cells is observed,and the expression levels of HMGB1 and RAGE are up-regulated.Butyrate can suppress the hyperglycemia-induced abnormal proliferation of NCM460 cells by down-regulating the HMGB1/RAGE signaling pathway.