安替可胶囊对Lewis肺癌小鼠肿瘤抑制作用及其抗血管生成作用的实验研究

The effects of Antike capsule in the treatment of Lewis lung cancer mice and its influence on the anti-angiogenesis effects in mice

目的:探讨安替可胶囊对Lewis肺癌小鼠的肿瘤抑制作用及其抗血管生成作用。方法:将40只C57BL/6小鼠分为模型组、阳性药组[索拉非尼0.16g/(kg·d)、低剂量组0.27g/(kg·d)及高剂量组0.54g/(kg·d)]。接种Lewis细胞进行造模,成功后对各组灌胃给予安替可,连续14d。末次给药24h后,观察各组体重、肿瘤体积、重量与肿瘤生长抑制率。对小鼠肿瘤组织进行病理组织学观测,并观察血管内皮生长因子(VEGF)、金属肽酶含血小板反应蛋白1(ADAMTS1)、低氧诱导因子-1α(HIF-1α)及血管内皮生长因子受体-2(VEGFR-2)免疫荧光染色表达量。结果:与模型组比较,低、高剂量组肿瘤体积与重量均明显降低,且体重明显增高(P<0.05),肿瘤生长抑制率分别为50.0%及40.6%。与模型组比较,高、低剂量组肿瘤组织中均可见不同程度的癌细胞出现坏死与凋亡。免疫荧光染色结果则表明,高、低剂量组VEGF、VEGFR-2、ADAMTS1及HIF-1α蛋白的相对表达量均明显优于模型组(P<0.05)。结论:安替可胶囊对Lewis肺癌小鼠具有较好的肿瘤抑制作用,其主要机制可能为抗血管生成活性。

Objective:To observe the effects of antike capsule in the treatment of Lewis lung cancer mice and its influence on the anti-angiogenesis effects in mice.Methods:40 Lewis lung cancer mice were divided into control group,sorafenib group[0.16 g/(kg·d)],high dose group[0.54 g/(kg·d)]and low dose group[0.27 g/(kg·d)].Using lewis lung cancer cells axillary vaccination to build the model,after the model built,the antike was gavaged for 14 days.24 h after the last gavage,the body weight,tumor volume and weight,tumor growth inhibition percent were observed.The histopathologies were observed in the mice,while immunofluorescent staining of VEGF,ADAMTS1,HIF-1αand VEGFR-2 were detected in the groups.Results:Compared with the model group,the body weight,the tumor volume and weight were significantly lower in high and low dose groups(P<0.05),while tumor growth inhibition percents were 50.0%and 40.6%.Pathological observations showed that the tumor cells in the high and low dose group were significantly necrosis and apoptosis in the tumor tissue than model group.Immunofluorescent staining shown,the expression of VEGF,ADAMTS1,HIF-1αand VEGFR-2 were significantly promoted in the high and low dose group than the model group(P<0.05).Conclusion:Antike capsule in the treatment of lewis lung cancer mice were effective,and have good anti-angiogenesis effects in mice.