性别对原发性高血压大鼠靶器官损伤及炎症因子的影响

Gender effects on target organ damage and inflammatory factors in spontaneous hypertensive rats

目的研究原发性高血压(EH)大鼠靶器官损伤及炎症因子的性别差异。方法选用6月龄原发性高血压大鼠(SHR),用无创尾动脉测压系统测定血压,用放射免疫分析法测定血浆血管紧张素Ⅱ(AngⅡ)、内皮素(ET-1)浓度,血清白介素-1β(IL-1β)、白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)和雌二醇(Estradiol,E2)浓度;测定心脏、血管和肾脏的器官指数,评价观察靶器官损伤。并以AngⅡ及ET-1与炎症因子做相关性分析。结果雌性SHR组的血压水平和血浆AngⅡ低于雄性组(P<0.05),ET-1高于雄性组(P<0.05);雌性SHR组的心室指数(VW/BW)、左心室指数(LVW/BW)大于雄性组(P<0.05);雄性SHR组的动脉血管指数血管指数(AW/L)及LVW/RVW大于雌性组(P<0.05)。雌性SHR组的血清白介素-1β高于雄性组(P<0.05),血清IL-6低于雄性组(P<0.05)。SHR血浆AngⅡ与血清炎症因子IL-1β及IL-6之间呈正相关,而与TNF-α之间没有相关性;ET-1与各炎症因子之间没有相关性。雌性SHR组血浆AngⅡ与血清E2之间呈正相关。结论 EH大鼠的血压水平、靶器官的损伤及炎症因子存在一定的性别差异性,Ang II是诱发高血压大鼠炎症反应的关键因素。

Objective To study the gender difference in target organ damage and inflammatory factors of the spontaneous hypertensive rats(SHR).Methods Blood pressure was measured by tail-cuff method(Non-invasive tail artery blood pressure measuring device),the concentration of AngiotensinⅡ(AngⅡ),endothelin-1(ET-1)in plasma and Estradiol(E2),Interleukin-1β(IL-1β),Interleukin-6(IL-6),tumor necrosis factor-α(TNF-α)in serum were detected by radioimmunoassay and target organ damage was observed in 6 months SHRs.Results The blood pressure level and plasma AngⅡin female SHR group were lower than that of male group(P<0.05)while ET-1 in females was significantly higher than that of male group(P<0.05).Ventricle weight/body weight(VW/BW),and left ventricle weight/body weight(LVW/BW)in female SHR group were significantly higher than that of male group(P<0.05).Arterial weight/length and right kidney weight/body weight(RKW/BW)in the male SHR group were significantly higher than that of the females group(P<0.05).The serum IL-1βin the female group was significantly higher than that of the male group(P<0.05).Serum IL-6 in the male SHR group was obviously higher than that of the females group(P<0.05),plasma AngⅡand serum inflammatory factors of IL-1βand IL-6 were positively correlated(P<0.05)in SHR,but there were no correlations between TNF-αand AngⅡand between ET-1 and inflammatory factors in SHR.plasma AngⅡwas positively correlated with serum E2 in the female SHR group(P<0.05).Conclusion The blood pressure level,target organ damage and inflammation factors in spontaneous hypertensive rats have certain gender differences,AngⅡis the main factor to induce inflammation of hypertensive rats.