冻干冷沉淀的制备与效果分析

Preparation and Effect Evaluation of Freeze-dried Cryoprecipitate

目的研究冷沉淀冻干保存的方法并评估其效果。方法共采集40例无偿献血者新鲜全血各200ml,每袋全血按常规方法制备并均分为容量相同的2袋冷沉淀,其中1袋置–20℃保存即为冰冻冷沉淀,另一袋加入复方氨基酸注射液后用冻干机制备成冻干冷沉淀,置于4℃保存。在保存24 h、12个月后各取20例献血者的两组冷沉淀,测定因子Ⅷ、纤维蛋白原含量和pH值。结果冻干冷沉淀与冰冻冷沉淀的因子Ⅷ、纤维蛋白原含量和pH值在保存24h、12个月时的差异均无统计学意义(P>0.05)。结论冻干冷沉淀在4℃保存12个月后仍然具有正常的因子Ⅷ、纤维蛋白原含量,可用于战备血液储备。

Objective To research the method of freeze-dried cryoprecipitate and evaluate its effect.Methods Forty units of fresh whole blood(200m l)were collected from volunteer donors.Two bags of cryoprecipitate with same capacity were prepared from each unit of whole blood by conventional method,one bag was stored at-20C as frozen cryoprecipitate,and the other was prepared into freeze-dried cryoprecipitate after adding Compound Amino Acid Injection by lyophilizer and stored at 4 C.Two sets of cryoprecipitates from 2 0 donors were taken out and analyzed for factorVⅢ.fibrinogen,and pH after 24 h,and the other cryoprecipitates were evaluated after 12 months.Results There are no significant differences of factorVⅢ,fibrinogen,and pH between frozen and freeze-dried cryoprecipitate after 24 hours,and 12 months storage,respectively(P>0.05).Conclusion The freeze-dried cryoprecipitate stored at 4C after 12 months remain normal factorVⅢand fibrinogen contents and can be used as blood reserve for wartime.