两种筛查献血者粒细胞抗癌活性方法的评价

An Evaluation of Two Methods for Screening Antineoplastic Activity of Granulocytes in Donor Cells

目的对两种筛查献血者粒细胞抗癌活性的方法进行评价。方法选择抗肿瘤综合基因检测和粒细胞体外杀伤试验方法进行"抗癌人"筛查;采集静脉血5 ml,肝素钠抗凝,681例的抗肿瘤综合基因检测由上海邃志生物科技有限公司在72 h内完成;811例的粒细胞体外杀伤试验由国跃生物科技(上海)有限公司在24 h内完成。对189例抗肿瘤综合基因检测的献血者进行粒细胞体外杀伤试验比对分析,对16例(首次基因检测为优秀、良好、中等、偏低各4例)献血者跟踪1月、3月后的基因检测和粒细胞体外杀伤试验情况。结果抗肿瘤综合基因检测评分"优秀"献血者占8.4%[(37+20)/681],"良好"献血者占11.0%[(45+30)/681];粒细胞体外杀伤试验"优秀"献血者占19.9%[(141+20)/811],与基因检测"优秀"和"良好"之和19.4%相近,基因得分与粒细胞体外杀伤试验评分大致相符。结论基因检测结果稳定,同一个体不同检测时间受外界干扰因素小、结果恒定,可能与基因检测与遗传信息有关,缺点是不能真实反映献血者当前的抗癌活性,筛查成本较高;粒细胞体外杀伤试验结果不稳定,同一个体不同时间的抗癌活性变化较大,可能与献血者心情、身体状况、季节变化等有关,优点是筛查成本较低,易于临床应用和推广。基因检测方法筛查献血者,由于结果为"优秀"的献血者较少,按ABO同型分布就更少,如仅选择评分"优秀"献血者,很难满足临床供应。可以将检测结果为"优秀"或"良好"的献血者按ABO血型同型建立献血者库,然后在采集前对献血者进行粒细胞体外杀伤试验,选择体外杀伤试验结果也为"优秀"的献血者进行单采粒细胞采集。

Objective To evaluate antineoplastic activity of granulocytes in blood donors screened by two different methods.Methods The anti-tumor genes were detected in 681 cases within 72 hours.The in vitro cytotoxicity assay of granulocytes and the anti-tumor gene test were carried out within 24 and 72 hours,respectively.811 cases were detected.The results of immunoprecipitation were compared with those of 189 blood donors who had been tested for anti-tumor gene.The results were compared in 1 6 patients(excellent,good,moderate and low in 4 cases,respectively).Blood donors were followed for 1 month and 3 months Of the gene detection and granulocyte in vitro killing test.Results The proportion of excellent blood donation was 8.4%and 11.0%was good.The excellent blood donation rate of granulocyte in vitro test was 19.9%,and the score of genetic testing was"Excellent"and"good"and 19.4%similar gene score and granulocyte in vitro killing test score roughly in line.Conclusion The result of genetic testing is stable,and the result is constant,which may be related to genetic information.The disadvantage is that it can not truly reflect the current anti-cancer activity of blood donors,and the cost of screening is high;the results of granulocyte in vitro killing test are unstable;The change of anticancer activity of the individual at different time may be related to the mood,physical condition and seasonal variation of blood donors.The advantage is that the screening cost is low and it is easy to be applied and popularized.