蛋鸡Ⅰ群腺病毒湖北株的分离与分子鉴定

ISOLATIONS AND CHARACTERIZATION OF GROUP Ⅰ ADENOVIRUS FROM SICK LAYING HEN IN HUBEI PROVINCE

从蛋鸡养殖场采集疑似"心包积水-肝炎综合症"蛋鸡的肝脏,分离病毒,处理后接种于SPF鸡胚。应用PCR方法扩增Ⅰ群腺病毒hexon基因并进行序列分析;将分离的病毒感染30日龄健康雏鸡,观察感染后鸡群临床症状和病死鸡病理变化。结果显示:分离的3株禽腺病毒病毒基因同源性为100%;与Ⅰ群腺病毒血清4型同源性为99.8%;与Ⅱ群禽腺病毒和Ⅲ群禽腺病毒同源性为43.1%~78.6%;病毒感染雏鸡后,第3 d出现鸡只死亡,第5 d达到死亡高峰期,整个试验阶段鸡只死亡率达到60%,解剖、死亡鸡可见典型心包积液-包涵体肝炎症状,肾细胞中出现空泡和坏死。结果表明,本研究成功分离1株Ⅰ群腺病毒,命名为Ⅰ群腺病毒湖北株。

Liver samples were collected from layers suspected"pericardial effusion and hepatitis syndrome"on different farms.The virus was isolated from the samples and inoculated with SPF chicken embryos after treatment.The viral DNA was extracted and amplifi ed in PCR for sequencing.Subsequently,30-day-old healthy chicks were inoculated with the isolated virus and clinical signs and pathological changes were observed.Three avian adenoviruses were isolated with 100%gene homology.These isolates shared 99.8%similarity of nucleotide sequences to those of serotype 4 of adenovirus group I and 43.1%-78.6%to those of adenovirus groupsⅡand III.Some of inoculated chicks died at day 3 post inoculation and the number of deaths peaked at day 5.Total chick mortality reached to 60%during the observation period.The typical signs of pericardial effusion and inclusion body hepatitis were observed.In addition,fatty and vacuolar degenerations were found in liver cells and vacuoles and necrosis in kidney cells.The results indicated that groupⅠAdenovirus was isolated from sick laying hen,named as Hubei strain.