亚麻主要农艺与品质相关性状的QTL定位

QTL Locations of Main Agronomic and Quality Traits in Flax

为了全面了解亚麻产量和品质相关性状的遗传基础,为亚麻基因克隆和分子标记辅助育种提供理论依据,在已构建SNP连锁遗传图谱的基础上,以LH-89为父本,R43为母本构建F2:3家系QTL定位群体,用R/QTL软件采用复合区间作图法对13个农艺和品质性状进行QTL定位。结果表明:(1)该研究共检测出35个QTL位点,与粗脂肪及其组成成分相关的QTL有20个,与农艺性状相关的QTL有15个;其中:亚油酸和粗脂肪各5个,亚麻酸、千粒重各4个,棕榈酸、株高、工艺长度各3个,硬脂酸、分枝数各2个,单株果数、果粒数、单株粒重、油酸各1个。(2)共有18个QTL的表型贡献率超10%(主效基因),其中农艺性状定位8个主效基因,品质性状定位10个主效基因。

In order to get more information about the genetic background of agronomic and quality traits of flax(Linum usitatissimum L.)for marker assistant selection(MAS)and map-based cloning,we constructed a F 2:3 QTL location population by using LH-89 and R43 as parents and made a quantitative trait locus(QTL)location study for 13 agronomic and quality traits based on previously constructed linkage genetic map by composite interval mapping(CIM)method and R/QTL programme.The results are as follows:(1)35 QTLs related to agronomic and quality traits in flax were detected.Five QTLs each for linoleic acid and oil content,four QTLs each for linoleic acid and thousand seeds weight,three QTLs each for palmitic acid,plant height,technical length,two QTLs each for stearic acid,branch number,one QTL each for bolls per plant,seeds per boll,seeds weight per plant and oleic acid.(2)Among them,18 QTLs which explained more than 10%of the phenotypic variation,i.e.,eight for agronomic traits and ten for quality traits,suggesting an existence of major-effect genes.