舌鳞状细胞癌细胞株CAL27外泌体源性miR-21、miR-221和miR-222的表达

Expression of miR-21, miR-221, and miR-222 in exosomes of CAL27 tongue squamous cell carcinoma cells

目的研究舌鳞状细胞癌细胞CAL27外泌体源性miR-21、miR-221和miR-222的表达。方法培养舌鳞状细胞癌细胞株CAL27和正常口腔上皮粘膜角质细胞HOK,收集培养上清液分离外泌体,电镜检测外泌体,qRT-PCR检测外泌体miR-21、miR-221和miR-222的表达。结果在舌鳞状细胞癌细胞CAL27和正常口腔上皮细胞HOK的培养基中都能检测出外泌体,qRT-PCR结果检测表明CAL27外泌体源性miR-21、miR-221和miR-222的表达比HOK升高(P<0.05)。结论舌鳞状细胞癌细胞外泌体源性miR-21、miR-221和miR-222的表达较正常口腔上皮细胞升高。

Objective To assess the expression of miR-21,miR-221,and miR-222 in exosomes of CAL27 tongue squamous cell carcinoma cells.Methods CAL27 tongue squamous cell carcinoma cells and normal human oral kerati-nocytes(HOKs)were cultured,and then,the cultured supernatant was collected to separate the exosomes.Exosomes were detected by electron microscopy,and the expression levels of miR-21,miR-221,and miR-222 in the exosomes of tongue cancer cells were measured by qRT-PCR.Results Exosomes existed in the cultured supernatants of CAL27 cells and HOKs.Additionally,the expression levels of miR-21,miR-221,and miR-222 in the exosomes of CAL27 cells were signifi-cantly enhanced compared with those in the HOK exosomes(P<0.05).Conclusion The expression levels of miR-21,miR-221,and miR-222 were markedly enhanced in the exosomes of CAL27 tongue squamous cell carcinoma cells.