摘要
目的探讨实时荧光定量聚合酶链反应(PCR)快速检测流行性脑脊髓膜炎(简称流脑)病原菌脑膜炎奈瑟菌(Nm)的临床价值。方法收集70例疑似流脑病例的双份脑脊液标本,1份采用细菌培养法进行检测,另1份采用实时荧光定量PCR进行检测,比较2种方法的检出阳性率以及敏感性和特异性。结果采用细菌培养法检出Nm阳性49例,阳性率为70.00%;实时荧光定量PCR检出Nm阳性67例,阳性率为95.71%,二者阳性率比较差异有统计学意义(P<0.05)。实时荧光定量PCR检测结果与细菌培养法阳性符合率为70.00%,其中有18例细菌培养法为阴性而实时荧光定量PCR为阳性。实时荧光定量PCR检测Nm的特异性为100%,无假阴性结果,阳性预测值为73.13%,阴性预测值为0.00%,检测下限为103拷贝/m L,线性范围为2×103~2×107拷贝/m L。结论实时荧光定量PCR检测Nm敏感性高、特异性强,且快速简便,为流脑流行病学调查提供了一种快速、简便的病原学诊断手段。
Objective To study the role of real-time fluorescence quantitation polymerase chain reaction(PCR)for Neisseria meningitidis(Nm)determination.Methods A total of 70 cerebrospinal fluid specimens were collected from patients with suspected meningitis.One part was performed bacterial culturing,and another part was determined by real-time fluorescence quantitation PCR.The positive rates,sensitivities and specificities of the 2 methods were compared.Results The positive rate of Nm by bacterial culturing was 70.00%(49 cases).Realtime fluorescence quantitation PCR showed a positive rate of 95.71%(67 cases).There was statistical significance for the positive rates between the 2 methods(P<0.05).The results of real-time fluorescence quantitation PCR and bacterial culturing showed a consistency rate of 70.00%.There were 18 cases being negative by bacterial culturing but being positive by real-time fluorescence quantitation PCR.The specificity of real-time fluorescence quantitation PCR was 100%,and no false negative results were obtained.The positive and negative predictive values were 73.13%and 0.00%,respectively.The lower limit of determination was 103 copies/mL.The linear range was 2×103-2×107 copies/L.Conclusions Real-time fluorescence quantitation PCR for Nm determination has high sensitivity and specificity and is rapid and convenient,which can be used for the etiological diagnosis of meningitis epidemiology.
作者
王崇义
史训忠
孙正林
WANG Chongyi;SHI Xunzhong;SUN Zhenglin(Department of Clinical Laboratory,the First Hospital of Ninghai County,Ningbo 315600,Zhejiang,China;Department of Clinical Laboratory,the Affiliated Hospital of Medical School of Ningbo University,Ningbo 315020,Zhejiang,China)
出处
《检验医学》
CAS
2018年第4期339-342,共4页
Laboratory Medicine
关键词
脑膜炎奈瑟菌
流行性脑脊髓膜炎
实时荧光定量聚合酶链反应
Neisseria meningitidis
Meningococcal meningitis
Real-time fluorescence quantitation polymerase chain reaction