氧化石墨烯/磷酸钙生物水泥体外生物相容性研究

In Vitro study on biocompatibility of graphene oxide/calcium phosphate cements

等摩尔的Ca_3(PO_4)2-CaHPO_4-CaCO_4(CPCs)水泥系为固相,不同质量浓度的氧化石墨烯(GO)水分散液(0~24mg/mL)为液相,按一定的固液比固化,得到复合材料GO/CPCs.以成骨细胞MC3T3-E1为对象,初步探究该复合材料试样的体外生物相容性.扫描电镜观察细胞贴壁形态,MTT法评价细胞活力,碱性磷酸酶(ALP)活性检测探讨材料的骨诱导性.结果显示:MC_3T3-E1细胞在材料表面状态良好,产生胞质突;GO的添加能够增强MC3T3-E1细胞活力;GO/CPCs在短期内(1d)促进MC3T3-E1细胞早期分化.GO/CPCs有望成为一种新型骨组织替代材料.

α-Ca 3(PO 4)2-CaHPO 4-CaCO 4(molar ratio1∶1∶1)and different concentrations of GO aqueous dispersion(0~24 mg/ml),dividedly used as the cement solid phase and the liquid component,are mixed at a certain powder/liquid ratio to set as GO/CPCs sample of composite cement.MC3T3-E1 cells are used to study the biocompatibility of GO/CPCs in vitro.The cell morphology on the surface of materials is observed by scanning electron microscopy.The viability of the cells is evaluated by MTT testing,and the bone-induction of cells is investigated by alkaline phosphatase(ALP)assay.The results show that the MC3T3-E1 cells get along well with GO/CPCs and have a cytoplasm-forming process.The activity of MC3T3-E1 cells and their differentiation at the short-term(1d)can be enhanced by GO/CPCs.The results observed above suggest that GO/CPCs may become a new material instead of bone tissue.