雷蒙德氏棉DNase Ⅰ高敏感位点(DHSs)文库构建方法

Construction of Gossypium raimondii DNase Ⅰ hypersensitive site library

染色质DNA对DNase Ⅰ表现出高敏感性的位点(DNase Ⅰ hypersensitive sites,DHSs)多是顺式作用元件所在的位置,包括启动子、增强子、调节子和衰减子等.研究DHSs位点的数目及其动态变化是探明顺式作用元件功能、揭示基因表达调控机制,乃至进行基因编辑和创新遗传材料的重要辅助手段.本文借鉴水稻和拟南芥DHSs文库构建方法,通过优化设计,初步建立了二倍体雷蒙德氏棉(Gossypium raimondii D5)的DHSs文库,为深入进行棉花功能基因组研究奠定基础.

DNase I hypersensitive sites(DHSs)usually are locations of the cis-regulatory elements on chromatin,such as promotors,enhancers,regulators and attenuators.Study on the number and dynamic changes of DHSs among the whole genome is an important auxiliary means to understand the function of cis-regulatory elements,reveal gene expression regulation mechanism,and even create new genetic material using genetic editing.Based on the construction of DHSs library in rice and Arabidopsis thaliana,DHSs library construction about diploid cotton Gossypium raimondii(D 5)was established,which will lay the foundation for further research on functional genomics in cotton.