摘要
Objective:This study aimed to evaluate the acute and chronic cytotoxicity and genotoxicity of 2,6-dichloro-1,4-benzoquinone(2,6-DCBQ).Methods:NIH3T3 cells were exposed to 2,6-DCBQ for 3 and 72 h,and relative cell viability was calculated.NIH3T3 cells were treated with different concentrations of 2,6-DCBQ for 24 h.The solvent and positive controls were dimethyl sulfoxide(DMSO)and 0.5μmol/L ethylmethylsulfone,respectively.The values of Olive tail moment were measured by comet assay.NIH3T3 cells were then simultaneously treated with 5μg/mL cytochalasin B and different concentrations of 2,6-DCBQ.The solvent and positive controls were DMSO and 1μmol/L mitomycin C,respectively.Micronucleus rates were calculated after 48 h.Results:The half lethal doses of 2,6-DCBQ in NIH3T3 cells were 64.93μmol/L for 3 hand 13.46μmol/L for 72 h.The values of Olive tail moment in the 7.5 and 10μmol/L groups were significantly higher than those in the solvent control(P<0.05).Moreover,the micronucleus rates in the 10 and 15μmol/L groups were significantly higher than those in the solvent control(P <0.05).The results of comet assay and micronucleus test showed a dose-response relationship.Conclusion:2,6-DCBQ exhibited strong cytotoxicity and induced DNA and chromosomal damage in NIH3T3 cells.
Objective:This study aimed to evaluate the acute and chronic cytotoxicity and genotoxicity of 2,6-dichloro-1,4-benzoquinone(2,6-DCBQ).Methods:NIH3T3 cells were exposed to 2,6-DCBQ for 3 and 72 h,and relative cell viability was calculated.NIH3T3 cells were treated with different concentrations of 2,6-DCBQ for 24 h.The solvent and positive controls were dimethyl sulfoxide(DMSO)and 0.5μmol/L ethylmethylsulfone,respectively.The values of Olive tail moment were measured by comet assay.NIH3T3 cells were then simultaneously treated with 5μg/mL cytochalasin B and different concentrations of 2,6-DCBQ.The solvent and positive controls were DMSO and 1μmol/L mitomycin C,respectively.Micronucleus rates were calculated after 48 h.Results:The half lethal doses of 2,6-DCBQ in NIH3T3 cells were 64.93μmol/L for 3 hand 13.46μmol/L for 72 h.The values of Olive tail moment in the 7.5 and 10μmol/L groups were significantly higher than those in the solvent control(P<0.05).Moreover,the micronucleus rates in the 10 and 15μmol/L groups were significantly higher than those in the solvent control(P <0.05).The results of comet assay and micronucleus test showed a dose-response relationship.Conclusion:2,6-DCBQ exhibited strong cytotoxicity and induced DNA and chromosomal damage in NIH3T3 cells.
出处
《广西医科大学学报》
CAS
2018年第3期281-285,共5页
Journal of Guangxi Medical University
基金
supported by grants from National Natural Science Foundation of China(No.81560524,81360421)
the China Postdoctoral Science Foundation (No.2013M540686, 2014T70839)
the Guangxi Natural Science Foundation(No.2012GXNSFBA053109)
the Outstanding Young Middle-aged Excellent Teachers’ Training in Higher Education Institutions of Guangxi
