树干注药对马尾松针叶几种生理指标和保护酶活性的影响

Effect of Trunk Injection of Pesticides on Physiological Properties and Protective Enzymes in Leaves of Pinus massoniana

2015年3月25日,以马尾松Pinus massoniana为材料研究氯氟氰虫酰胺和甲维盐树干注药后其针叶内几种生理指标和保护酶活性的变化。结果表明:2种药剂树干注药后均可导致马尾松针叶内叶绿素和可溶性总糖含量下降,而丙二醛(MDA)含量上升,其中氯氟氰虫酰胺的影响较为显著,叶绿素a在注药后4 d下降了10.3%,可溶性总糖在注药后8 d下降了15%,MDA在注药后4 d时均上升了42%。2种药剂树干注药后针叶内超氧化物歧化酶(SOD)活性均呈上升-下降-上升-下降的过程,氯氟氰虫酰胺对SOD活性影响较显著,注药后4 d活性上升了39.5%;而苯丙氨酸解氨酶(PAL)活性均呈上升-下降的过程,氯氟氰虫酰胺对PAL活性影响较显著,注药后8 d活性上升了40.7%。

Injection of Cyhalodiamide and Emamectin benzoate in Pinus massoniana trunk on March 25th of 2015 in Jiangxi province for determination of physiological properties and protective enzymes in leaves of tested trees.The result showed that content of chlorophyll and total soluble sugar of injected trees decreased,but that of MDA increased.Trees injected by Cyhalodiamide had decrease of chlorophyll content by 10.3%in the 4th day,of total soluble sugar content by 15%in the 8th day,while of MDA content increase by 42%in the 4th day.The activity of SOD in trees injected the two pesticides had a curve of up-down-up-down.Cyhalodiamide had significant effect on SOD and PAL activities,the activity of SOD increased 39.5%in the 4th day,and that of PAL 40.7%in the 8th day after trunk injection.