改进的QuEChERS方法快速测定鸡肉中5种磺胺

Rapid Analysis of Five Sulfonamides in Chicken by Using An Improved QuEChERS Method

建立一种改进的QuEChERS结合UPLC-MS/MS方法测定鸡肉中的5中磺胺。鸡肉样品经乙腈水溶液提取、超声和离心后,加入C_(18)净化,离心后氮气吹干。经UPLC-MS/MS分析,溶剂和基质标液的r均在0.9990以上,鸡肉中5种磺胺在高中低3个浓度的回收率为70.1%~85.6%,日内和日间精密度分别在2.2%~11.1%和2.6%~11.8%。LOD和LOQ分别在0.231~0.349μg·kg^(-1)和0.700~1.057μg·kg^(-1),方法的基质效应较小。因此,本方法具有简单、快速和灵敏的特点,并成功运用于分析鸡肉样品中的5种磺胺。

A method was developed for determining five sulfonamides in chicken using improved QuEChERS and ultra-performance liquid chromatography coupled to tandem quadrupole mass spectrometry(UPLC-MS/MS).The acetonitrile/waters were added to the chicken samples,subjected to UAE and then centrifuged.The supernatant was taken for the C 18 clean-up.The centrifuged supernatant was evaporated to dryness under gentle ultra-high-purity nitrogen gas.Analysis was performed by ultra-performance liquid chromatography coupled to tandem quadrupole mass spectrometry(UPLC-MS/MS).All solvent and matrix-matched calibration curves showed excellent correlation coefficient>0.9990.The recoveries were between 70.1%and 85.6%in chicken matrices studied at three spiked levels of low,medium,and high concentrations and the range of RSD of intra and inter day were 2.2%~11.1%and 2.6%~11.8%,respectively.The limits of detection(LODs)and limits of quantification(LOQs)were 0.231~0.349μg·kg-1 and 0.700~1.057μg·kg-1,respectively.A weak matrix effect was observed.The proposed method was proven very simple,fast and sensitive,and was successfully applied for the analysis of five Sulfonamides in chicken samples.