HPLC-ELSD法测定复胃散胶囊中黄芪甲苷的含量

Determination of astragaloside Ⅳ in Fuweisan Capsules by HPLC-ELSD

目的建立高效液相色谱-蒸发光散射检测器法测定复胃散胶囊中黄芪甲苷的含量。方法采用Agilent 1200高效液相色谱仪和Waters Xselect C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-水(32∶68),流速为1.0 m L·min-1,进样量为20μL,蒸发光散射检测器(漂移管温度为70℃,载气流速为2.0 L·min-1)。结果黄芪甲苷在0.050 5~1.011 mg·m L-1的范围内呈良好的线性关系,标准曲线为lg A=1.355 5lg C+3.277 6(r=0.999);精密度和重复性的RSD分别为1.2%和1.6%;平均回收率为96.1%,RSD为1.7%。结论该方法简便准确、快速、方便、精密度高、重复性好,可用于复胃散胶囊中黄芪甲苷的含量测定。

Objective To establish a HPLC-ELSD method for the determination of astragalosideⅣin Fuweisan Capsules.Methods The analysis was performed on a Waters XSELECT C18 column(4.6 mm×250 mm,5μm)with the mobile phase of acetonitrile-water(32:68)at a flow rate of 1.0 mL·min-1.The injection volume was 20μL.The temperature of the drift tube was 70℃and the flow rate of carrier gas was 2.0 L·min-1.Results The linearity equation of astragalosideⅣwas lgA=1.3555lgC+3.2776,(r=0.999)and the linear range was 0.050 5~1.011 mg﹒mL-1.Precisions and repeatabilities of the methods were 1.2%and 1.6%.The average recovery rate was 96.3%with RSD=1.9%.Conlusion The method was accurate,rapid,reproducible and reliable,which can be used for the determination of astragalosideⅣin Fuweisan Capsules.