凤头白鸭与连城白鸭的群体遗传多样性分析

Genetic Diversity Analysis of Crested White Duck and Liancheng Duck Populations

为系统地评价、保护凤头白鸭资源的遗传多样性,进一步对其开发利用,本研究利用12个微卫星位点(STR分型技术)对凤头白鸭与连城白鸭(各60只)2个群体的遗传多样性参数进行检测和计算。结果表明:除位点AJ272578以外,凤头白鸭群体在APL2、AJ272577、AJ272579、AJ515884、APH01、AY493256、AY4932898、CMO11等8个STR位点上的等位基因多于连城白鸭,在AJ515887、AJ515893、AY493313等3个位点上与连城白鸭相等;凤头白鸭群体的有效等位基因数(Ne)、期望杂合度(He)、观察杂合度(Ho)、多态信息含量(PIC)均大于连城白鸭;2个群体的群体内近交系数(Fis)分别为-0.02、0.09,凤头白鸭群体表现为杂合子过剩。综上,凤头白鸭群体的遗传多样性较连城白鸭更为丰富。

The detected and analyzed result of 12 microsatellite loci in Crested white ducks and Liancheng white ducks indicated that eight microsatellite loci(APL2,AJ272577,AJ272579,AJ515884,APH01,AY493256,AY4932898,CMO11)of Crested white ducks possess more alleles than that of Liancheng white ducks,three microsatellite loci(AJ515887,AJ515893,AY493313)equally and one single polymorphic(AJ272578)less.The average of Ne,He,Ho and PIC in Crested white ducks is larger than in Liancheng white ducks.Besides,the Fis of Crested white ducks and Liancheng white ducks is-0.02 and 0.09 respectively,indicate heterozyosis excess in Crested white ducks.The overall results showed that Crested white ducks have higher genetic diversity than Liancheng white ducks in the 12 microsatellite loci.