中国荷斯坦奶牛EEF1D基因的SNPs检测及其与产奶性状的关联分析

SNP Detection of EEF1D Gene in Chinese Horstein Cow and Its Correlation Analysis with Milk Production Traits

试验旨在研究中国荷斯坦奶牛真核生物翻译延伸因子1 D(eukaryotic translation elongation factor 1delta,EEF1D)基因的多态性及其与产奶性状的相关性。利用Sequenom MassARRAY SNP分型技术对宁夏地区1 252头中国荷斯坦奶牛EEF1 D基因的多态性进行了检测,并对其多态位点不同基因型和组合基因型与产奶性状进行了关联分析。结果显示,EEF1 D基因的5′侧翼区存在2个SNPs位点,即EEF1D-1和EEF1D-3;经检测发现,EEF1D-1存在2种基因型,EEF1D-3存在3种基因型。χ2检验表明,中国荷斯坦奶牛在EEF1D-1位点偏离Hardy-Weinberg平衡状态(P<0.05),在EEF1D-3位点未偏离Hardy-Weinberg平衡状态(P>0.05);EEF1D-1和EEF1D-3位点多态信息含量(PIC)分别为0.10和0.28,分别呈现低度多态和中度多态。在试验群体中,EEF1D-1位点对乳脂率和乳蛋白率性状的效应均达到极显著水平(P<0.01),对305d产奶量性状的效应达到显著水平(P<0.05);EEF1D-3位点对305d产奶量、乳脂率和乳蛋白率性状的效应均达到极显著水平(P<0.01);EEF1 D基因的优势基因型组合GG-AG和GG-GG个体乳脂率均显著高于GG-AA组合个体(P<0.05)。说明EEF1 D基因可以作为影响中国荷斯坦奶牛产奶性状的候选基因用于标记辅助选择。

This study was aimed to investigate the SNP of eukaryotic translation elongation factor 1 delta(EEF1D)gene in Chinese Horstein cow and it’s correlation analysis with milk production traits.Sequenom MassARRAY SNP genotyping method was applied to detect the polymorphisms of EEF1D gene in 1 252 Chinese Holstein cows,and the association of EEF1D genotype and genotype combination with milk production traits were analyzed.The results showed that two SNPs were identified,including EEF1D-1 and EEF1D-3 in 5′-flanking region.Two genotypes were found in EEF1D-1 and three genotypes were found in EEF1D-3.Chi-square test showed that EEF1D-1 locus deviated from Hardy-Weinberg equilibrium in Chinese Holstein cow(P<0.05),but EEF1D-3 locus did not deviate from Hardy-Weinberg equilibrium in Chinese Holstein cow(P>0.05).Polymorphic information content(PIC)in EEF1D-1 and EEF1D-3 loci were 0.10 and 0.28,showing low polymorphism and moderate polymorphism.In the EEF1D-1 locus,it had extremely significant and significant effect on milk fat percentage and milk protein percentage(P<0.01)and 305 d milk yield traits(P<0.05),respectively.In the EEF1D-3 locus,it had extremely significant effects on 305 d milk yield traits,milk fat percentage and milk protein percentage(P<0.01).The GG-AG and GG-GG genotypes combination in Chinese Holstein cow had significantly greater milk fat percentage than those with GG-AA(P<0.05).Therefore,EEF1D gene might be useful as molecular markers for milk yield traits in Chinese Holstein cow breeding.