妊娠糖尿病患者血清中RBP4及NF-κB活性的变化及临床意义

Changes of serum RBP4 and NF-κB in patients with gestational diabetes mellitus and its clinical significance

目的探究视黄醇结合蛋白4(RBP4)和蛋白质核因子κB(NF-κB)在妊娠糖尿病患者外周血中的表达及其临床意义。方法选取2014年12月-2016年12月在该院产科产检的104例孕妇,依据其糖耐量检测结果分为妊娠糖尿病(GDM)组46例和妊娠正常糖耐量(NGT)组58例,通过ELISA测定两组血清中RBP4、NF-κB、空腹血糖(FBG)及空腹血胰岛素(Fins)等指标的变化,并分析其与胰岛素抵抗(HOMAIR)的相关性。结果妊娠糖尿病组血清中RBP4、NF-κB、FBG及HOMA-IR等指标均高于妊娠正常糖耐量组(P<0.05)。血清中NF-κB的活性与HOMA-IR呈正相关(r=0.755,P=0.003);血清中RBP4的水平与HOMA-IR不相关(r=0.268,P=0.376);RBP4与NF-κB的水平呈正相关(r=0.752,P=0.003)。结论RBP4水平和NF-κB活性在妊娠糖尿病的发生、发展中起重要作用,可能为其治疗提供潜在的靶点。

Objective To explore the clinical significance of the RBP4 and NF-κB in serum of patients with gestational diabetes mellitus.Methods Totally 104 pregnant women were collected in our hospital from December 2014 to December 2016,which were divided into gestational diabetes mellitus group(GDM)(46 cases)and normal glucose tolerance pregnancy group(NGT)(58 cases)according to the sugar tolerance test results.Changes of serum RBP4 level,NF-κB activity,fasting blood glucose(FBG)and fasting blood insulin(Fins)were detected by Enzyme-linked immunosorbent assay(ELISA).Correlations between insulin resistance(HOMA-IR)and them were analyzed.Result Compared with the GNT group,serum RBP4 level,NF-κB activity,FBG level and Fins level were significantly increased in GDM group(P<0.05).The serum NF-κB activity was positively correlated with HOMA-IR(r=0.755,P=0.003);there was no significant correlation between the serum BRP4 level and HOMA-IR(r=0.268,P=0.376);the serum BRP4 level had a positive correlation with NF-κB activity(r=0.752,P=0.003).Conclusion BRP4 level and NF-κB activity may play an important role in gestational diabetes mellitus process,and they are likely to be potential targets for treatment of gestational diabetes mellitus.