生物相溶性多孔炭-香豆素复合材料的制备及其细胞成像和靶向给药(英文)

Synthesis of a biocompatible nanoporous carbon and its conjugation with florescent dye for cellular imaging and targeted drug delivery to cancer cells

将棕榈叶在惰性气氛中经一步热解过程制备出多孔炭(NPCs),并结合香豆素-6(C-6)形成NPCs-C-6复合荧光染料来进行癌细胞成像及靶向给药。通过XRD和Raman分析可知,NPCs具有高的含碳量。傅里叶红外分析表明棕榈叶中含有纤维素、半纤维素和木质素,这有利于NPCs的形成,以及香豆素-6为导航分子的细胞成像和靶向给药。采用SEM和TEM观察NPCs结合前后的结构形貌,XPS表征元素组成。采用Zeta电位为初始指标来确定颗粒分散质量,以适合生物医学应用。NPCs-C-6分析细胞毒性和成像试验表明,对正常细胞系(MDCK:ATCCCCL-34^(TM))有高的生物相溶性,对人体癌细胞系(A-375∶ATCCCRL-1619TM与N2A∶ATCCCCL-131^(TM))有高的毒性。因此,这些研究结果有望为生物基功能NPCs作治疗载体的研究进展提供新思路。

A nanoporous carbon(NPC)was synthesized from Oil palm leaves(OPL)by pyrolysis at 600℃in a N 2 atmosphere,oxidized by a mixture of sulfuric and nitric acids(3∶1 vol/vol)and conjugated with coumarin-6 as a fluorescence dye for cellular imaging and drug delivery to cancer cells.The structure,morphology and dispersion stability of NPC in aqueous media before and after the conjugation were investigated by XRD,FT-IR,Raman spectroscopy,SEM,TEM and zeta potential measurement.Cell uptake for the conjugated NPC was investigated by fluorescence microscopy.Results indicate that the cellulose,hemicelluloses and lignin in OPL convert to a graphitic structure by pyrolysis.NPC consists of spherical nanoparticles with diameters of 30-50 nm and has a high graphitic content with an I D/I G ratio of 0.7.The coumarin-6 is successfully conjugated to NPC by forming a complex.Both NPC and the conjugated NPC have a high dispersion stability in aqueous media.NPC is biocompatible with a negligible cytotoxicity.The conjugated NPC exhibits a high cell uptake,is highly biocompatible for normal cells and toxic for tested human cancer cells.NPC is a good candidate for cellular imaging and targeted drug delivery.