量子点荧光探针检测结直肠癌中的p53和EGFR共表达

Co-expression of p53 and EGFR in colorectal cancer detected by quantum dots fluorescence probe

目的利用量子点(QDs)荧光探针同时检测结直肠癌组织标本中的两种肿瘤标志物p53、表皮生长因子受体(EGFR)。方法 QDs荧光探针检测结直肠癌中p53和EGFR的共表达,并对TNM分期、分化程度等因素进行统计学分析。结果 p53在结直肠癌组织中强阳性率为48.6%,与N分期显著相关(P<0.05),EGFR和p53共表达在结直肠癌组织中的阳性率为67.1%,与N分期显著相关(P<0.05),与其他因素无关(P>0.05)。结论 QDs法有助于定量分析p53和EGFR在结直肠癌中的协同表达,可为临床上结直肠癌的预后提供依据。

Objective Simultaneous detection of two tumor markers p53 and EGFR in colorectal cancer samples.Methods Co-expression of p53 and EGFR in colorectal cancer detected by QDs fluorescence probe.TNM staging,grade and other factors were analyzed.Results The strong positive rate of p53 in colorectal cancer was 48.6%.It was significantly correlated with the N staging(P<0.05),the positive rate of EGFR and p53 in colorectal carcinoma was 67.1%.It was significantly correlated with the N staging(P<0.05),and was independent of other factors(P>0.05).Conclusion The method of QDs is helpful to quantitative analysis of co expression of p53 and EGFR in colorectal cancer,and it can provide the basis for clinical prognosis of colorectal cancer.