乳源免疫调节肽对小鼠急性酒精性肝损伤的保护作用

Protective effect of immunomodulating peptide( PGPIPN)derived from beta-casomorphin in bovine milk on acute alcohol-induced liver injury

目的研究乳源免疫调节肽对小鼠急性酒精性肝损伤的保护作用。方法将60只清洁级♂昆明种小鼠随机分为正常组、模型组、乳源免疫调节肽(PGPIPN)低、中、高剂量组和阳性对照谷胱甘肽(GSH)组。连续灌胃2周,最后3 d,采用白酒(56度)给小鼠连续灌胃建立急性酒精性肝损伤模型,测定各组小鼠体质量、肝脏指数及相关生化指标;取肝脏制作组织切片HE染色,观察肝组织的病理学变化。结果模型组小鼠血清中谷丙转氨酶(alanine aminotransferase,ALT)和谷草转氨酶(aspartate aminotransferase,AST)明显高于正常对照组,肝组织出现了肝索排列紊乱,肝细胞坏死,炎性细胞浸润。与模型组相比,PGPIPN高剂量组肝脏指数、血清ALT、AST水平较模型组明显降低。PGPIPN高剂量组TNF-α含量较模型组明显减少,肝组织中谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、超氧化物歧化酶(superoxidedismutase,SOD)活性增强,丙二醛(malondialdehyde,MDA)水平明显下降;肝组织病理损伤也得到明显改善。结论 PGPIPN对小鼠急性酒精性肝损伤具有较好的保护作用。

Aim To investigate the protective effect of immunomodulating peptide(PGPIPN)on the acute alcoholic liver injury in mice.Methods Kunming mice were randomly divided into control group,model group,glutataione(GSH)group,PGPIPN low dose group,PGPIPN moderate and high dose groups.The mice were treated with different doses of PGPIPN or GSH for two weeks except control group and model group.The acute alcoholic liver injury model was induced by gavage with 56°alcohol for three days.The indices including the activities of AST,ALT in serum,and the contents of TNF-α,MDA,SOD and GSH-Px in liver were examined.Liver histopathological changes were examined by HE staining.Results Compared with control group,the levels of serum ALT,AST and the contents of TNF-α,MDA significantly increased,while the contents of SOD and GSH-Px significantly decreased in model group.There was hepatocyte apoptosis and inflammatory cell infiltration in liver tissues.Compared with model group,the activities of serum ALT,AST and the contents of TNF-α,MDA were remarkably reduced in PGPIPN high dose group.The contents of SOD and GSH-Px significantly increased in PGPIPN high dose group.PGPIPN could alleviate the injury of liver.Conclusion PGPIPN has certain protective effect on acute alcoholic liver injury of mice,providing a theoretical guidance.