使用全自动生化分析仪检测尿香草扁桃酸的性能评价

Evaluation of detection performance of urinary vanilmandelic acid on automatic biochemical analyzer

目的:评价使用全自动生化分析仪检测尿香草扁桃酸(VMA)的性能指标,并与手工法相比较。方法:分别使用基于均相酶免疫分析技术的全自动生化分析仪(即上机法)和手工法检测VMA的正确度、精密度、线性范围、回收率和抗干扰能力等指标;并使用2种方法对高血压、甲状腺功能亢进、甲状腺功能减退、待确诊患者和表面健康受试者的尿液标本进行检测试验,评价其阳性率等指标。结果:2种方法检测VMA的正确度、精密度、线性范围、回收率和抗干扰能力等指标均达到了试剂盒说明书给出的标准,但上机法的上述指标优于手工法。上机法临床标本检测的总体阳性率和手工法大体相似,但对检测结果处于临界值处患者诊断更有优势。结论:上机法检测VMA较传统的手工法具有更好的性能和更大的临床应用价值,值得进行更大范围的临床应用实验。

Objective To evaluate the automatic biochemical analyzer when used to detect urinary vanilmandelic acid(VMA),and to compare it with manual method.Methods The automatic biochemical analyzer using homogenous enzyme immunoassay technology was compared with the manual method on accuracy,precision,linear range,recovery rate,anti-interference capability and etc when used to detect VMA.The comparison was also carried out on positive rate and etc when the two methods were used to test the urine specimens of the healthy subjects and suspected patients of hypertension,hyperthyroidism and hypothyroidism.Results The two methods both had the results on accuracy,precision,linear range,recovery rate,anti-interference capability meet the requirements described in the instruction of reagent kit,while the analyzer gained advantages over the manual method.The positive rates by the two methods for testing urine specimens were similar,while the analyzer behaved better in diagnosing the patient with critical value.Conclusion The analyzer proves better than the manual method when used to detect VMA,and thus is worthy promoting in clinical trial.