ZBTB20在SLE患者外周血B细胞中的表达和临床意义

Expression of zinc finger protein ZBTB20 in peripheral blood B cells in patients with SLE and its clinical significance

目的研究锌指和BTB结构域蛋白20(ZBTB20)在系统性红斑狼疮(SLE)患者外周血B细胞中的表达,并探讨ZBTB20在SLE中的临床意义。方法采用RT-PCR技术检测36例SLE患者(SLE组)和30例健康对照者(对照组)外周血CD19^+B细胞中ZBTB20 mRNA的表达水平;Western blot检测分析外周血ZBTB20蛋白水平;流式细胞术检测B细胞亚群所占比例。分析SLE患者B细胞中ZBTB20mRNA的表达与B细胞亚群比例变化的关系及与临床指标(抗ds-DNA抗体、抗核抗体、免疫球蛋白IgG、抗ENA抗体)的相关性。结果 SLE组外周血CD19^+B细胞中ZBTB20mRNA的表达明显高于对照组(P<0.05);SLE组患者外周血ZBTB20蛋白水平高于对照组(P<0.05)。与对照组相比,SLE患者外周血B细胞亚群,CD19^+B细胞比例降低(P<0.05),CD19^-CD138^+浆细胞/CD19^+B细胞之间的比值及CD19^-CD138^+浆细胞比例明显增高(P<0.05);SLE患者B细胞中ZBTB20mRNA的表达水平与CD19^-CD138^+浆细胞/CD19^+B细胞的比值呈正相关(P<0.05);ZBTB20mRNA与抗ds-DNA抗体、免疫球蛋白IgG、抗核抗体、抗ENA抗体均呈正相关(P<0.05)。结论 ZBTB20可能通过促进B细胞分化从而参与SLE发病。

Objective To study the expression of zinc finger and BTB domain containing 20(ZBTB20)in peripheral blood B cells of the patients with systemic lupus erythematosus(SLE),and to investigate its clinical significance in SLE.Methods The ZBTB20 mRNA expression level in peripheral blood CD19+B cells of 36 cases of SLE(SLE group)and 30 healthy controls(control group)was detected by RT-PCR.The ZBTB20 protein level was detected by western blot;the proportion of B cell subset was measured by flow cytometry.Then the relationship between the ZBTB20 mRNA expression with B cells subset proportion change and its correlation with clinical indicators[anti-dsDNA antibody,immunoglobulin G(IgG),anti-nucleosome antibody(ANA)and anti-extractable nuclear antigen(ENA)antibody]in SLE patients were analyzed.Results The expression level of ZBTB20 mRNA in peripheral blood CD19+B cells of the SLE group was significantly higher than that of the control group(P<0.05).The peripheral blood ZBTB20 protein level was higher than that of the control group(P<0.05);the peripheral blood B cells subsets and CD19+B cells proportion in the SLE patients were decreased,while the CD19-CD138+plasmocytes/CD19+B cells ratio and CD19-CD138+plasmocytes proportion were significantly increased(P<0.05).The expression level of ZBTB20 mRNA in B cells of SLE patients was positively correlated with the ratio of CD19-CD138+plasmocytes/CD19+B cells(P<0.05).The expression of ZBTB20 mRNA was positively correlated with anti-ds-DNA antibody,ANA and anti-ENA antibody(P<0.05).Conclusion ZBTB20 might participate in the pathogenesis of SLE possibly via promoting B cells differentiation.