Notch3、DLL1、CD133在结直肠腺癌中的表达及意义

Expression and significance of Notch3,DLL1 and CD133 in colorectal adenocarcinoma

目的探讨Notch3、DLL1、CD133在结直肠腺癌中的表达及其临床病理意义。方法运用免疫组织化学法检测12份正常结直肠黏膜标本、30份结直肠腺瘤组织标本、50份结直肠腺癌组织标本,了解Notch3、DLL1、CD133在不同结直肠组织中的表达情况,分析其与临床病理特征间的关系。结果 Notch3、DLL1、CD133在结直肠腺癌中的阳性表达率分别为64.0%(32/50)、68.0%(34/50)和54.0%(27/50),均明显高于在结直肠腺瘤中的阳性表达率(26.7%、33.3%、36.7%)和正常结直肠组织中的阳性表达率(8.3%、16.7%、8.3%)(P<0.05)。腺瘤组与正常组相比,差异均无统计学意义(P>0.05)。以上指标在结直肠腺癌中的表达与患者年龄、性别、肿瘤发生部位、分化程度、肿瘤大小无关(P>0.05),均与是否伴淋巴结转移相关,且Notch3和DLL1的表达与Dukes分期相关,此外DLL1的表达与肿瘤浸润深度也相关(P<0.05)。Notch3与CD133的表达呈正相关(r=0.478,P=0.000)。结论在结直肠腺癌组织中Notch3、DLL1、CD133的表达明显高于正常结直肠组织及结直肠腺瘤组织;Notch3可能通过对肿瘤干细胞的调控,影响结直肠癌的发生、发展。

Objective To investigate the expression of Notch3,DLL1 and CD133 in human colorectal adenocarcinoma and its clinical pathology meaning.Methods Immunohistochemical staining was used to detect the expression of Notch3,DLL1 and CD133 in 12 cases of normal colorectal mucosa tissue,30 cases of colorectal adenoma tissue and 50 cases of colorectal adenocarcinoma tissue,and the relationship between them and clinicopathological data were analyzed.Results The positive rates of Notch3,DLL1 and CD133 were 64.0%(32/50),68.0%(34/50)and 54.0%(27/50)in colorectal carcinoma tissues,respectively,which were remarkably higher than those in colorectal adenoma tissue(26.7%,33.3%,36.7%)and those in normal colorectal mucosa tissue(8.3%,16.7%,8.3%)(P<0.05).There was no significant difference between the adenoma group and the normal group(P>0.05).The expression levels of Notch3,DLL1 and CD133 were not correlated to age,gender,tumor location,degree of differentiation and the tumor size,except lymph node metastasis,in addition,the expression of Notch3 and DLL1 were also associated with Dukes staging,and The expression of DLL1 were also associated with tumor infiltration depth(P<0.05).The expression of Notch3 protein was positively related to that of DLL1 protein(r=0.478,P=0.000).Conclusion The positive expression rates of Notch3,DLL1 and CD133 in colorectal adenocarcinoma tissue are remarkably higher than those in the normal colorectal mucosa and colorectal adenoma tissue,which prompts that the high expression of Notch3,DLL1 and CD133 may all participate in the process and metastasis of colorectal adenocarcinoma.Moreover Notch3 signalling pathway may act through the cancer stem cells and eventually regulate the initiation and development of colorectal adenocarcinoma.