Cdk5及p35在星形胶质细胞正常及缺血再灌注后的表达研究

The Expression of Cdk5 and p35 in Primary Cultured Astrocytes and the Changes in Expression after Ischemia/Reperfusion

目的:研究细胞周期蛋白依赖性激酶(Cdk)5和p35在星形胶质细胞中的表达及缺血再灌注后的其表达变化趋势。方法:离体培养SD大鼠星形胶质细胞,通过免疫荧光染色观察Cdk5和p35在星形胶质细胞中的表达分布。对培养的星形胶质细胞进行糖氧剥夺/恢复(OGD/R)处理,Western blot检测Cdk5和p35在星形胶质细胞OGD/R后不同时间点的表达变化。结果:Cdk5和p35均在星形胶质细胞中存在表达,以胞浆为主,胞核表达较少;两者表达分布一致。OGD/R后Cdk5和p35的表达呈早期显著上升,后期下降的趋势。结论:Cdk5和p35在星形胶质细胞中均存在表达,以胞浆为主,且两者表达分布基本一致。OGD/R后星形胶质细胞中的Cdk5和p35的表达呈动态变化。

Objective:To investigate the expression of Cdk5 and p35 in primary cultured astrocytes and the changes in expression induced by ischemia/reperfusion.Methods:Astrocytes were cultured from SD neonate rats.The expression and distribution of Cdk5 and p35 in cultured astrocytes were analyzed by immunofluorescence staining.Primary cultured astrocytes were subjected to oxygen-glucose deprivation/reperfusion(OGD/R),and the changes in Cdk5 and p35 expression after OGD/R were observed by Western blot.Results:Cdk5 and p35 were expressed in astrocytes,mainly in the cytoplasm and less in the nucleus,and both displayed similar cellular distribution.The expression of Cdk5 and p35 in astrocytes was increased during early phases and decreased during late phases after OGD/R.Conclusion:Both Cdk5 and p35 were expressed in astrocytes,and both showed similar,mainly cytoplasmic distribution.Cdk5 and p35 expression in astrocytes exhibited dynamic changes after OGD/R.