应用定量蛋白质组学对谷子干旱响应蛋白的研究

Studies on Drought-Responsive Proteins of Foxtail Millet by Applying Quantitative Proteomic

谷子是我国的传统优势作物,具有抗旱、耐瘠薄、高光效以及基因组小、生育期短等突出优势,受到国际遗传学界的高度重视,已迅速发展成为功能基因组研究新的候选模式作物。但目前在蛋白质水平上解析谷子干旱响应的研究还较少。本研究利用TMT(tandem mass tags)体外同重同位素标记的相对与绝对定量技术,筛选并鉴定谷子干旱响应蛋白。研究发现谷子干旱响应差异蛋白主要参与胁迫响应、碳代谢、光合作用和蛋白合成等相关代谢途径。该研究对于深入解析谷子的抗旱机制,培育抗旱耐逆谷子新品种具有重要意义。

Foxtail millet is the traditional dominant crop in China.It has many outstanding advantages including drought resistance,poor soil tolerance,high photosynthetic efficiency,small genome and short growth period.The international field of genetics has paid more attention to it.It has been rapidly developed as the new candidate mode crop to study functional genomes.But there were less studies on the drought response of foxtail millet from protein level analysis at present.In the study,the drought response proteins of foxtail mille were screened and indentified by TMT technology.The results found that differential proteins in drought response of foxtail mille mainly participated in related metabolic pathways including stress response,carbon metabolism,photosynthesis and protein synthesis.This study was of great significance to deeply analyzing drought resistance mechanism and breeding new varieties of foxtail millet with drought and adversity resistance.