菜豆普通花叶病毒辽宁分离物的鉴定与序列分析

Identification and Sequence Analysis of Bean common mosaic virus in Liaoning

2016年8月,于辽宁省盖州市采集到表现为黄化的菜豆样品。利用Potyvirus通用引物Legpoty-F/Legpoty-R对3个代表样品进行RT-PCR扩增,得到预期725 bp的片段。Blast显示它们与菜豆普通花叶病毒(Bean common mosaic virus,BCMV)同源性最高。利用报道的特异引物BCMV-CP-F/BCMV-CP-R扩增到约1.2 kb的片段,包含完整的外壳蛋白(coat protein,cp)。基于cp基因进行同源性分析,发现它们与美国菜豆分离物BCMV-[USA:Iowa:Phaseolus vulgaris:11](KM023744)同源性最高,为97.9%。根据Potyvirus的分类标准,认为它们是BCMV的辽宁分离物。这是辽宁首次报道菜豆感染BCMY。

Samples from the Phaseolus vulgaris L.showing yellow symptoms were collected from Gaizhou,Liaoning Province,in August 2016.The universal degenerate primers LegpotyF/LegpotyR were used for the detection of Potyviruses from three symptomatic leaf samples by RT-PCR.The expected fragments 725 bp in size were amplified.Blast results showed that they shared the highest identities with Bean common mosaic virus(BCMV).With the reported specific primers BCMV-CP-F/ZYMV-CP-R,the expected fragments about 1.2 kb which contained complete coat protein gene(cp)was obtained.Based on nucleotide sequence of cp,the homology analysis results showed that they had the highest homology with the USA isolate BCMV-[USA:Iowa:Phaseolus vulgaris:11](KM023744),for 97.9%.According to the classification criteria of Potyvirus,they are considered as the Liaoning isolate of BCMV.This is the first report of the natural occurrence of BCMV on Phaseolus vulgaris L.in Liaoning.