载抗菌肽KSL对钛合金种植体体内骨结合的影响

The Effect of Antibacterial Peptide KSL on Bone Binding in the Implant of Titanium Alloy

目的:为进一步提高钛合金口腔种植体的植入成功率,缩短愈合时间,减少各种并发症的发生率,引入抗菌肽KSL观察其骨结合能力。方法:钛合金种植体微弧氧化膜层(A组)、微弧氧化-硅烷复合膜层(B组),微弧氧化-硅烷-抗菌肽KSL复合膜层(C组)。将三组种植体分别植入家兔下颌骨中2周、4周、8周,采用锥形束CT(CBCT)、扭力实验、扫描电镜及能谱分析检测种植体-骨界面成骨情况。结果:CBCT观察随着植入时间的增加,种植体周围尤其是种植体-骨界面处的低密度影逐渐缩小,C组表现最为明显;扭力实验C组的骨结合力是A组的2倍,B组介于两者之间。扫描电镜观察植入8周,C组新生骨带最宽,骨结合最好,Ca/P比最高,已有部分新生骨转化为成熟骨,且C组不同时间点的新生骨均多于A、B组。结论:以硅烷为偶联剂载抗菌肽KSL的钛合金微弧氧化种植体具有较高的骨结合能力,并能促进早期骨形成。

Objective:In order to further improve the implant success rate,shorten the healing time and reduce the incidence of complications,antibacterial peptide KSL is introduced to observe the bone binding ability.Methods:Microarc oxide film layer of titanium alloy implant(group A),microarc oxidation-silane composite membrane layer(group B)and microarc oxidation-silane-antibacterial peptide KSL composite membrane layer(group C).The 3 groups of implants are implanted into the mandible of rabbits for 2 weeks,4 weeks and 8 weeks respectively.The conical beam CT(CBCT),torsion test,scanning electron microscope and energy spectrum analysis are used to detect the osseous condition of implant bone interface.Results:According to the CBCT,with the prolongation of implantation time,the low density shadow around the implant,especially at the implant bone interface shrinks,and the C group shows the most obvious.The torsion test shows that the bone resultant force in group C is 2 times higher than that in group A,and group B is between the two.After 8 weeks of scanning electron microscope,the new bone band in group C is widest,the combination of bone is best and the Ca/P ratio is highest.Some new bone is transformed into mature bone,and the new bone in C group is more than that in group A and B at different time points.Conclusion:The bone binding ability of titanium alloy microarc oxidizing implants for silane coupling agent containing plant body antibacterial peptide KSL,which can promote early bone formation.