正交试验优化超声波辅助提取石榴皮渣中果胶

Optimization of Ultrasonic-Assisted Extraction of Pectin from Pomegranate Pomace by Orthogonal Experiments

以石榴(Punica granatum L.)皮渣为原料,超声波辅助酸溶醇沉提取果胶,优化超声辅助提取石榴果胶的条件,为进一步开发利用石榴加工副产物提供依据。在单因素基础上,采用正交试验分析提取温度、提取时间、料液比、水酸比对果胶得率的影响。结果表明,在超声波频率为45 GHz下,提取温度55℃,提取时间75 min,水酸比为6∶1(m L∶m L)加入2 mol/L的亚硫酸,料液比1∶50(g∶m L),果胶得率达到34.41%。超声波辅助法提取石榴皮渣中果胶,得率较高,有开发价值。

Ultrasonic-assisted extraction of acid-solution and alcohol precipitation was applied to extract pectin from pomegranate(Punica granatum L.)pomacel.The optimum conditions of ultrasonic-assisted extraction of pectin from pomegranate pomacel were determined and provided basis for further exploitation of by-products in pomegranate processing.Based on single factor test,orthogonal experiments were designed to optimize the influences of extracting temperature,extracting time,the ratio of solid to liquid and water to acid on the pectin yield.Results showed that at the ultrasonic frequency of 45 GHz,extracting temperature of 55℃,an extraction time of 75 min,water-acid ratio of 6∶1(mL∶mL),the add of sulphurous acid of 2 mol/L,solid-liquid ratio of 1∶50(g∶mL),the yield of pectin was 34.41%.By the study of ultrasonic-assisted extraction of pectin from pomegranate pomace,the extraction rate was high and pomegranate pomace had very high value of development and utilization.