牡丹子叶离体再生研究

Study on Regeneration System for Cotyledons in Peonyin Vitro

以牡丹(Paeonia suffruticosa)品种凤丹的子叶片为起始外植体,研究了不同种类、浓度及其配比的植物生长调节剂对牡丹愈伤组织诱导、不定芽分化及不定根诱导等离体再生过程的影响。结果表明,0.1%升汞浸泡7 min,再用10%次氯酸钠溶液浸泡5 min消毒效果最佳,胚的无菌率和萌发率分别为82.96%、56.03%;诱导愈伤组织的最佳NAA浓度为3.0 mg/L,出愈率达85.03%;最适宜的不定芽诱导培养基为WPM+1.0 mg/L 6-BA+0.3 mg/L NAA,不定芽诱导率可达77.35%,平均不定芽数为3.05个;最适宜的不定芽伸长培养基为WPM+0.3 mg/L 6-BA+2.0 mg/L GA3,不定芽伸长率为81.12%,芽的平均伸长长度可达1.93 cm;最适宜的生根培养基为1/2MS+2.0 mg/L NAA+1.5 mg/L IBA+1.0 mg/L Ag NO3,生根率达54.31%。

The cotyledon leaves of peony(Paeonia suffruticosa)cultivars Fengdan was used as the initial explants,the effects of different species,concentration and compatibility of plant hormones on the regeneration process of peony cotyledon leaves were studied,including callus induction,adventitious bud differentiation and adventitious root induction.The results showed that the use of 0.1%mercury immersion for 7 minutes,then 10%sodium hypochlorite solution soak for 5 minutes is the best disinfection method,aseptic rate and germination rate of embryo were 82.96%and 56.03%.The optimum naphthylacetic acid(NAA)concentration in callus occurrence was 3.0 mg/L and the callus induction rate was 85.03%.The optimum medium for the adventitious buds occurrence was WPM+1.0 mg/L 6-benzylamino purine(6-BA)+0.3 mg/L NAA along with the frequency of differentiation was 77.35%and average number of adventitious buds was 3.05.The suitable medium for adventitious bud elongation was WPM+0.3 mg/L 6-BA+2.0 mg/L gibberellin(GA3).The elongation efficiency was about 81.12%,and the average shoot length was 1.93 cm.The optimum rooting medium was 1/2MS+2.0 mg/L NAA+1.5 mg/L indole butyric acid(IBA)+1.0 mg/L AgNO3,the rooting rate reached 54.31%.