用流化制粒技术制备的愈肠颗粒的药效学研究

Pharmacodynamic study of Yuchang granules prepared by fluidized granulation technology

目的:观察愈肠颗粒对2,4,6-三硝基苯磺酸(TNBS)诱导的慢性溃疡性结肠炎模型大鼠的药效学。方法:采用流化制粒技术制备愈肠颗粒。建立TNBS诱导的慢性溃疡性结肠炎大鼠模型。72只大鼠按体重分层随机分成6组:正常对照组、模型组、阳性对照肠炎宁(10g/kg)组和愈肠颗粒5、10、15g生药/kg组,灌胃给药,qd,共10d。记录给药第2、4、6、8、10天(d 10)的体重及d 1、d 3、d 5、d 7、d 9、d 10腹泻及便血的大鼠数,并进行结肠的大体及组织病理学检查。结果:与正常对照组比较,模型组大鼠体重增加量减少,均出现便血和腹泻,结肠损伤以及结肠病理评分较正常对照组高,差异有显著意义(P<0.01)。肠炎宁组大鼠d 2、d 4、d 6、d 8、d 10体重增加量明显超过模型组(P<0.01),d 7、d 9、d 10便血大鼠数明显减少(P<0.05),d 7腹泻大鼠数明显减少(P<0.05),结肠损伤评分以及结肠组织病理学评分比模型组明显降低,差异有显著意义(P<0.01)。愈肠颗粒15g生药/kg组大鼠体重变化量明显超过模型组(P<0.01),d 7、d 9便血大鼠数明显减少(P<0.05),d 7腹泻大鼠数明显减少(P<0.05)。愈肠颗粒10、15g生药/kg组大鼠结肠损伤评分以及结肠组织病理学评分均比模型组明显降低(P<0.01),此作用具有剂量依赖性,愈肠颗粒15g生药/kg的作用与肠炎宁10g/kg相当。结论:愈肠颗粒能够减少发生便血和腹泻的慢性溃疡性结肠炎模型大鼠的数量,剂量依赖性减轻结肠损伤程度,促进溃疡愈合。

Objective:To investigate the pharmacodynamics of Yuchang granules in the rat model of chronic ulcerative coli-tis(CUC)induced by 2,4,6-three nitrobenzene sulfonic acid(TNBS).Methods:Yuchang granules were prepared by fluidized granulation technology.A rat model of CUC was set up by TNBS.According to body mass stratification,72 rats were random-ly divided into 6 groups:the normal control group,the model group,the Changyanning 10 g/kg group(as a positive control)and the Yuchang granules 5,10,15 g crude drug/kg groups.The drugs were administered by gavage once daily for 10 days.The body mass on days 2,4,6,8,10 and the numbers of the rat with diarrhea and hematochezia on days 1,3,5,7,9,10 were recorded.The visual and histopathological examinations of the colon were performed as well.Results:As compared with that of the normal control group,the weight gain of the model group decreased,hematochezia and diarrhea occurred in all the rats of the model group,and the scores of colon lesion and colon histopathology were all higher than those of the rats in the normal control group.Statistical significance could be seen,when comparisons were made between the 2 groups(P<0.01).The weight gain at 2,4,6,8,10 days in the rats of the Changyanning group was significantly greater than that of the rats in the model group,the number of the rats with hematochezia at 7,9,10 days was significantly less(P<0.05),the number of the rats with diarrhea at 7 days significantly decreased(P<0.05),and the scores of colon lesion and colon histopathology all de-ceased as compared with those of the model group.Statistical significance could also be noted,when comparisons were made between the 2 groups(P<0.01).Changes in the body mass of the rats in the Yuchang granules 15 g crude drug/kg group were obviously greater than those of the rats in the model group(P<0.01),the number of the rats with hematochezia at 7 and 9 days obviously decreased(P<0.05)and the number of the rats with diarrhea at 7 days also obviously decreased(P<0.05).The scores of colon lesion and colon histopathology for the rats in the 10 or 15 g crude drug/kg Yuchang granules groups all dose-dependent trend(P\0.01),and the effect of 15 g crude drug/kg Yuchang granules was equivalent to that of the effect of 10 g crude drug/kg Changyanning granules.Conclusion:Yuchang granules could decrease the rates of hematochezia and diarrhea in the model rats with CUC,anddose-dependently alleviate colon lesion and promote ulcer healing.