水蜡树果实总黄酮提取工艺优化及其抗氧化活性

Process Optimization and Antioxidant Activity of Flavonoids Extracted from Fruits of Ligustrum obtusifolium

为研究水蜡树果实总黄酮超声辅助提取的最优工艺并探讨其体内抗氧化活性,以水蜡树果实为原料,乙醇体积分数、液料比和超声时间为自变量,水蜡树果实总黄酮得率为因变量,运用响应曲面法优化水蜡树果实总黄酮提取工艺,同时通过灌胃试验测定水蜡树果实总黄酮对小鼠肝组织中丙二醛(MDA)水平和超氧化物歧化酶(SOD)活性的影响。结果表明水蜡树果实总黄酮的最优提取工艺为:液料比11∶1(mL∶g)、超声时间11min和乙醇体积分数60%,在此条件下,总黄酮的得率为(4.83±0.03)mg·g^(-1);水蜡树果实总黄酮具有良好的抗氧化活性,可明显降低小鼠体内肝组织的MDA水平,并显著提高其SOD活性。说明响应曲面法优化水蜡树果实总黄酮提取工艺方法简便,预测性较好,且提取的总黄酮具有良好的体内抗氧化活性。

An ultrasound-assisted process to extract flavonoids from the fruits of Ligustrum obtusifolium was optimized,and the antioxidant activity of the extract analyzed in vivo.Based on a single factor test on ethanol concentration,solvent∶substrate ratio,and ultrasonic application duration,the extraction procedure was optimized using response surface methodology based on flavonoid yield as the indicator.With an in vivo feeding,the level of malondialdehyde(MDA)and activity of superoxide dismutase(SOD)in livers of the test mice were determined.The optimal processing conditions,including a solvent∶substrate ratio of 11 mL∶1 g,an ultrasonic application for 11 min,and the use of 60%ethanol for solvent,rendered a flavonoid yield of(4.83±0.03)mg·g-1 for the extraction.The extract obtained significantly suppressed MDA production and raised SOD activity in livers of the mice.