声光可调-近红外光谱法快速测定丹参药材中隐丹参酮的含量

Rapid Determination of Cryptotanshinone in Salvia miltiorrhiza by AOTF-NIDRS

目的:建立快速测定丹参药材中隐丹参酮含量的方法。方法:采用高效液相色谱法测定药材样品中隐丹参酮含量(作为参考值)。采用声光可调-近红外光谱法结合偏最小二乘法建立药材样品中隐丹参酮含量的定量模型:根据药材样品中隐丹参酮含量测定结果,采集35份药材样品,以一阶导数法联合平滑滤波系数法预处理光谱,药材样品中隐丹参酮含量测定最佳波段范围为1 250~2 150 nm。结果:药材样品中隐丹参酮含量测定方法学经验证符合要求。隐丹参酮定量模型的校正均方根偏差为0.014 6,预测均方根偏差为0.022 3,相关系数为0.976 6。内部验证偏差为2.41%,外部验证偏差为4.06%。结论:该方法快速准确,简便无污染,可用于丹参药材中隐丹参酮含量的快速测定。

OBJECTIVE:To establish rapid method for content determination of cryptotanshinone in Salvia miltiorrhiza.METHODS:The content of cryptotanshinone in sample was determined by HPLC(as reference value).AOTF-NIDRS combined with PLS was used to establish quantitative correction model for the content of cryptotanshinone in S.miltiorrhiza.According to the results of content determination of cryptotanshinone in samples,35 samples of medicinal material were collected.First-order derivative combined with smoothing filter coefficient method was used to pretreat spectrum,and optimal band range for content determination of cryptotanshinone in sample ranged 1 250-2 150 nm.RESULTS:Methodology validation of content determination of cryptotanshinone in sample was in line with the requirements.Correction mean square deviation of quantitative correction model of cryptotanshinone was 0.014 6,and predicted mean square deviation was 0.022 3,coefficient of association was 0.976 6.The internal verification deviation was 2.41%and the external verification deviation was 4.06%.CONCLUSIONS:This method is rapid,accurate,simple and pollution-free.It can be used for rapid content determination of cryptotanshinone in S.miltiorrhiza.