瑶药破骨风的水提工艺优化及其水提物的抗炎镇痛作用研究

Study on the Optimization of Water-extraction Technology of Yao Medicine Jasminum lanceolarium and the Anti-inflammatory and Analgesic Effect of Its Water Extract

目的:优化瑶药破骨风的水提取工艺,并考察其水提物的抗炎镇痛作用。方法:采用单因素试验和正交试验,以阿魏酸含量、干膏率为指标,考察提取时间、液料比、提取次数3个因素对水提取工艺的影响,优选最佳工艺,并进行验证试验。以阿司匹林为阳性对照,采用二甲苯致小鼠耳廓肿胀法和角叉菜胶致小鼠足肿胀法观察低、中、高剂量(3、6、12 g/kg,以生药计)的破骨风水提物的抗炎作用,采用热板致小鼠疼痛反应和醋酸致小鼠扭体反应考察其镇痛作用;采用酶联免疫吸附法(ELISA)测定小鼠以角叉菜胶致炎后血清中肿瘤坏死因子α(TNF-α)和白细胞介素1β(IL-1β)的含量,采用紫外分光光度法检测小鼠血清和炎性组织中前列腺素E2(PGE2)的含量。结果:最优提取工艺为提取时间120 min,液料比16∶1(m L/g),提取3次。与模型组比较,破骨风水提物低、中、高剂量组小鼠耳廓肿胀度、足肿胀率均显著降低,扭体次数显著减少,疼痛反应痛阈值显著升高(P<0.05或P<0.01);与模型组比较,该水提物低、中、高剂量组小鼠血清中的TNF-α、IL-1β、PGE2含量与炎性组织中的PGE2含量均显著减少(P<0.05或P<0.01)。结论:优选的瑶药破骨风水提工艺效率高、稳定可行;其水提物对模型小鼠具有明显的抗炎镇痛作用,可能是通过降低体内TNF-α、IL-1β、PGE2水平发挥作用。

OBJECTIVE:To optimize the water-extraction technology of Yao medicine Jasminum lanceolarium and study the anti-inflammatory and analgesic effect of its water extract.METHODS:The single factor test and orthogonal test were used to investigate the effects of extraction time,liquid-solid ratio and extraction times on water-extraction technology and optimize extraction technology using ferulic acid content and dry extract yield as index.The validation test was also conducted.With aspirin as positive control,the method of ear edema induced by xylene and paw edema induced by carrageenan in mice were used to observe the anti-inflammation effects of low-dose,medium-dose and high-dose(3,6,12 g/kg,by crude drug)of J.lanceolarium water extract;the analgesic effect of those was investigated by hot-plate induced pain response test and mice writhing response test.The contents of TNF-αand IL-1βwere determined by ELISA after carrageenan induced inflammation in mice.The contents of PGE2 in serum and inflammation tissue were detected by UV spectrophotometry.RESULTS:The optimal extraction technology was as follows as extraction time of 120 min,liquid-solid ratio of 16∶1(mL/g),extracting for 3 times.Compared to model group,the ear edema degree and paw edema rate of mice were raduced,writhing response times were lessened,and pain response threshold was enhanced significantly in low-dose,medium-dose and high-dose groups of J.lanceolarium water extract(P<0.05 or P<0.01).Compared to model group,the contents of TNF-α,IL-1βand PGE2 in serum and the content of PGE2 in inflammation tissue of mice were raduced significantly in low-dose,medium-dose and high-dose groups of J.lanceolarium water extract(P<0.05 or P<0.01).CONCLUSIONS:The optimized water-extraction technology of Yao medicine J.lanceolarium shows high efficiency,stable and feasible.Water extract shows significant anti-inflammatory and analgesic effects through reducing the contents of TNF-α,IL-1βand PGE2.