强心安神汤对慢性心衰大鼠AngⅡ及AT1mRNA表达的影响

Effects of Qiangxin Anshen Decoction on the Expression of AngⅡ and AT1mRNA in Rats with Chronic Heart Failure

目的研究强心安神汤对Wistar慢性心衰模型大鼠AngⅡ含量及AT1 m RNA表达的影响,探讨其可能的作用机制。方法除空白组外,将Wistar大鼠造模后分为模型组、西药组、强心安神汤高剂量组及强心安神汤低剂量组,干预4周后,抽静脉血以免疫组化法测定大鼠血清中AngⅡ的含量;处死大鼠,提取心肌组织,以RT-PCR法检测心肌组织AT1 m RNA的表达。结果强心安神汤组大鼠血清AngⅡ含量及AT1 m RNA表达与模型组及西药组比较,差异有统计学意义(P<0.05或P<0.01)。结论强心安神汤治疗心衰的机制可能与其抑制AngⅡ引起的AT1 m RNA表达上调有关。

Objective To study the effect of Qiangxin Anshen decoction on the expression of Ang域and AT1 mRNA in the model of Wistar rats with chronic heart failure,and investigate its possible mechanism.Methods Except for the blank group,the model rats were randomly divided into model group,Western medicine group,high-dose Qiangxin Anshen decoction group and low-dose Qiangxin Anshen decoction group.After intervention treatment for 4 weeks,the content of Ang域of serum in rats was measured.The rats were killed and the AT1 mRNA expression in myocardial tissues were detected.Results Compared with the model and Western medicine groups,the content of Ang域in the rats serum and AT1 mRNA expression of myocardial tissues in Qiangxin Anshen decoction group was statistically significant(P﹤0.05 or P<0.01),Conclusion The mechanism of Qiangxin Anshen decoction in treating heart failure may be related with the up-regulation of AT1 mRNA expression caused by restraining Ang II.