Wnt通路抑制剂ETC-159处理对口腔鳞癌细胞增殖迁移的影响

Effects of Wnt pathway inhibitor ETC-159 on proliferation and migration of OSCC cells

目的研究Wnt通路抑制剂ETC-159对口腔鳞癌细胞系(SCC-15)增殖迁移的影响并探讨其机制。方法分别用DMSO和ETC-159处理SCC-15细胞12 h和24 h,用CCK8试剂盒检测细胞增殖活性,用Transwell小室检测细胞迁移能力,用Western blot法检测Wnt信号通路相关蛋白Wnt3a、β-catenin含量及Wnt通路调节的细胞增殖迁移相关蛋白cMyc、cyclin D1、CD146含量。结果 ETC-159组处理SCC-15细胞12 h和24 h后,其增殖活性和迁移能力及Wnt3a、β-catenin、c-Myc、cyclin D1、CD146表达量均显著下降,与DMSO组相比差异具有统计学意义(P<0.05)。结论 Wnt信号通路抑制剂ETC-159能通过降低c-Myc、cyclin D1、CD146的水平并抑制SCC-15细胞的增殖和迁移。

Objective The objective of this study was to investigate the effect of Wnt pathway inhibitor-ETC-159 on proliferation and migration of oral squamous cell carcinoma(SCC-15)cells and to explore its mechanism.Methods SCC-15 cells were treated with DMSO and ETC-159 for 12 h or 24 h.Cell proliferation was detected by CCK8 kit.Transwell assay was used to detect the ability of cell migration.Western blotting was used to detect cell migration related to proteins i.e.Wnt3a and p-catenin,proliferation and migration related proteins i.e.c-Myc,cyclin D1,CD146.Results After treated with ETC-159 for 12 or 24 h,th e proliferation,migration and expression of W nl3a,p-catenin,c-Myc,cyclin D1 and CD 146 in SCC-15 cells were significantly decreased when compared to the DMSO group(P<0.0 5).Conclusion Wnt signaling pathway inhibitor-ETC-159 can inhibit the proliferation and migration of SCC-15 cells by decreased levels of c-Myc,cyclin D1 and CD146.