中国地鼠口腔颊黏膜癌中miRNA与mRNA表达谱的关联分析

Association of expression profiles of miRNA and mRNA in Chinese hamster buccal pouch squamous cell carcinoma

目的构建中国地鼠口腔颊黏膜癌(OSCC)中差异表达的miRNA和mRNA表达谱,初步预测与OSCC发生发展相关的miRNA和mRNA。方法运用新一代高通量测序技术构建miRNA和mRNA表达谱,通过Gene Ontology功能富集和KEGG Pathway分析预测与OSCC发生发展相关的miRNA和mRNA。结果成功构建中国地鼠口腔颊黏膜癌中差异表达的miRNA和mRNA表达谱,共发现11个已知miRNA、3个新miRNA显著性差异表达和194个mRNA差异表达,且一个miRNA可调控多个mRNA,多个miRNA靶向调控某个mRNA。结论本实验OSCC中差异表达的miRNA通过调控mRNA形成复杂的调控网络,在口腔癌的发生发展中发挥重要作用,为口腔癌的发生、发病机制,及临床治疗及预后判断提供理论依据。

Objective To establish the expression profiles of differentially expressed miRNA and mRNA in oral squamous cell carcinoma(OSCC)and to investigate the roles of miRNA and mRNA associated with the occurrence and development of OSCC.Methods The expression profiles of miRNA and mRNA were constructed using a new generation of high-throughput sequencing techniques.The miRNA and mRNA associated with the occurrence and development of OSCC were predicted by Gene Ontology enrichment analysis and KEGG pathway analysis.Results We successfully constructed the differentially expressed miRNA and mRNA profiles of Chinese hamster buccal pouch squamous cell carcinoma.11 known and 3 novel significantly differentially expressed miRNAs and 194 differentially expressed mRNAs were found.A miRNA can regulate multiple mRNAs,and multiple miRNAs can control one mRNA.Conclusions Differential expression of miRNA play a an important role in the carcinogenesis and development of OSCC through regulating mRNA and forming a complex regulatory network.It provides theoretical data for the occurrence,pathogenesis,clinical treatment and prognosis of OSCC.