维吾尔医异常黑胆质证大鼠模型的尿液代谢组研究

Urine metabonomics study of abnormal Savda syndrome rat model in Uyghur traditional medicine

目的用外部特征观察与尿液代谢组研究相结合的方式,对维吾尔医异常黑胆质证大鼠模型进行生物学标志评价与讨论。方法采用维吾尔医异常黑胆质理论建立大鼠动物模型,并对其各项外部指标如毛发、舌象、睡眠、粪便、情绪、体重增长率等进行观察和评分,采用核磁共振氢谱(~1H-nuclear magnetic resonance,~1HNMR)对其尿液进行检测与分析。结果异常黑胆质证模型组大鼠与对照组相比较外部特征发生了明显的变化,包括粪便干燥且硬,尿量减少且颜色加深,皮毛干燥,舌苔暗紫并有瘀斑,体重增长率降低等;尿液代谢物中23种代谢物含量明显降低包括丙酸酯、乳酸、丙氨酸、丙酮酸、乙酸、乙酰胺、糖蛋白、丙酮、甲胍、肌氨酸、鸟氨酸、甘氨酸、肌酸、肌酸酐、肌胺酸酐、β-半乳糖、尿刊酸酯、酪氨酸、苯丙氨酸、马尿酸、氨基马尿酸、甲酸和赖氨酸等,而尿素、柠檬酸、尿囊素和α-酮戊二酸等4种代谢物含量明显升高。结论模型组大鼠在异常黑胆质证发生发展过程中不但外在表形发生明显变化,而且也发生了内在多种代谢通路的明显变化,尿液代谢产物的明显异常可能与异常黑胆质证的生物学机制有关。

Objective To evaluate and discuss the changes of biomarkers of abnormal Savda syndrome rat model in Uyghur traditional medicine by external feature observation and urine metabolomics assessment.Methods The abnormal Savda syndrome rat model was established according to the theory of Uyghur traditional medicine.Its external characteristics such as hair,tongue,sleep,feces,emotion and weight growth rate were observed and scored,and their urine was detected and analyzed by proton nuclear magnetic resonance(1H-NMR)spectroscopy.Results Compared with the healthy control group,there were significant changes in the external features in the abnormal Savda syndrome rat model group,including dry and hard stools,reduced urine output and darker color,dry fur,dark purple tongue with ecchymosis,and decreased weight growth rate.Moreover,23 urinary metabolites were significantly reduced,including propionate,lactic acid,pyruvic acid,acetic acid,alanine,acetamide,glycoprotein,acetone,methyl guanidine,sarcosine,ornithine,glycine,creatine,creatinine,aminoanhydride,β-galactose,urocanate,tyrosine,phenylalanine,hippuric acid,aminohippuric acid,formic acid and lysine.However,urea,citric acid,allantoin andα-ketoglutaric acid were significantly increased.Conclusions During the development process of Savda syndrome,there are not only abnormal changes in external appearance in the model rats,but also evident changes of many internal metabolic pathways.The obvious abnormalities of the urine metabolites may be related to the biological mechanisms of abnormal Savda syndrome.