噪声污染对大鼠血激素和Hsp-70水平的影响

Effect of noise pollution on serum hormone and Hsp-70 levels in rats

目的探讨噪声污染对大鼠血激素水平和热休克蛋白-70(Hsp-70)表达的影响。方法 40只雄性Wistar大鼠随机分为对照组(正常饲养)和实验组(分为35、65、85 d B三个组),每组10只动物。每天刺激1次,每次刺激30 min,连续刺激20 d。第21天采血用酶联免疫吸附法(ELISA)检测血液中去甲肾上腺素(NA),睾酮(T),多巴胺(DA),Hsp-70水平。结果 ELISA法检测发现,实验组(35、65、85 d B组)大鼠体重比对照组分别减轻了23.45%,30.13%,35.64%,T和DA水平分别降低了9.12%、20.06%、37.99%和15.49%、18.31%、24.88%;血NA和Hsp-70水平分别升高了35.08%、171.52%、197.86%和39.34%、195.09%、285.25%,差异均有显著性(均P<0.01)。结论噪声污染能显著影响大鼠血激素水平和热休克蛋白-70的表达。

Objective To study the effect of noise pollution on serum hormone and heat shock protein-70(Hsp-70)levels in rats.Methods Forty male Wistar rats were randomly divided into control group(normal),experimental group(further divided into 35,65 and 85 dB three groups),each group 10 animals,stimulated for 30 min once a day,continually stimulated for consecutive 20 days.On the 21st day of experiment,the serum noradrenaline(NA),testosterone(T),dopamine(DA)and Hsp-70 levels were determined by enzyme-linked immunosorbent assay(ELISA).Results Compared with the control group,the body weight of experimental group(35,65 and 85 dB groups)was reduced by 23.45%,30.13%,and 35.64%,respectively The serum T and DA levels were decreased by 9.12%,20.06%,37.99%and 15.49%,8.31%,24.88%,respectively;while the serum NA and Hsp-70 levels were increased by 35.08%,171.52%,197.86%,and 39.34%,195.09%,and 285.25%,respectively.All the result showed a significant difference(P<0.01).Conclusions Noise pollution can significantly affect the serum levels of hormone and heat shock protein-70 expression in rats.