摘要
目的探索一种最适合初学者的快速、方便和无血污染的SD大鼠脑脊液收集方法。方法取雄性SD大鼠45只,分成三组:第一组,经肌肉穿刺延髓池抽取法,第二组,经皮直接穿刺延髓池抽取法,第三组,直视下经硬脊膜穿刺延髓池抽取法。结果第一组共有13只大鼠成功获得80~140μL的脑脊液,2只采集到血性脑脊液,成功率为87.6%;第二组共成功抽取脑脊液7只,成功率为46.7%;第三组共成功抽取脑脊液5只,成功率为33.3%。结论经肌肉穿刺延髓池抽取脑脊液法是一种适合初学者的方便快捷、无污染的SD大鼠脑脊液采集技术。
Objective To explore a rapid,convenient and non-polluting method of collecting cerebrospinal fluid(CSF)in Sprague Dawley(SD)rats for beginners.Methods Forty-five SD rats were divided into three groups-group 1(collecting CSF through neck muscles),group 2(collecting CSF through skin directly),and group 3(collecting CSF through endorhachis).Results The successful rate of the CSF collection in the group 1 was 86.7%,group 2 was 46.7%,and group 3 is 33.3%.Conclusions The modified collection method of CSF by cisterna puncture through neck muscles in SD rats is simple,convenient,efficient and non-polluting,and is especially suitable for beginners.
作者
朱奕昕
叶冬青
李晓莉
ZHU Yixin;YE Dongqing;LI Xiaoli(Department of Neurology,Affiliated Zhongda Hospital of Southeast University,Nanjing 210009,China;The Institute of Neuropsychiatric Research of Southeast University,Nanjing 210009)
出处
《中国比较医学杂志》
CAS
北大核心
2018年第4期113-115,共3页
Chinese Journal of Comparative Medicine
基金
国家自然科学基金青年项目(81501173)
关键词
大鼠
脑脊液
采集技术
rats
cerebrospinal fluid
collection method
