橘皮素对泌乳素腺瘤细胞增殖和凋亡的作用及其机制研究

Effects of Tangeretin on Proliferation and Apoptosis of Prolactinoma MMQ Cells and Its Mechanism in vitro

目的观察橘皮素对泌乳素腺瘤MMQ细胞增殖和凋亡的影响。方法 MTS试验检测不同浓度橘皮素处理泌乳素腺瘤MMQ细胞24、48、72 h后细胞增殖活力。不同浓度的橘皮素处理MMQ细胞72 h后经Annexin V-FITC/PI双染,流式细胞仪分析细胞凋亡的情况。Western印迹检测橘皮素处理MMQ细胞72 h后bcl-2和bax凋亡相关蛋白表达水平影响。结果不同浓度的橘皮素能够以时间和浓度依赖性地抑制MMQ细胞的增殖,并能够显著诱导MMQ细胞凋亡。橘皮素处理72 h后能够显著下调MMQ细胞中抗凋亡蛋白bcl-2的蛋白表达,同时上调促凋亡蛋白bax的表达。结论橘皮素可能通过bcl-2/bax信号通路诱导泌乳素腺瘤MMQ细胞凋亡,从而抑制细胞增殖。

Objective To study the effects of tangeretin inhibiting the proliferation and inducing apoptosis of prolactinoma MMQ cells.Methods After treated with different concentration of tangeretin for 24,48,72 h,the proliferation of prolactinoma MMQ cells was determined by MTS.Simultaneously,the apoptosis ratio of MMQ by Annexin V-FITC/PI and the expression of bcl-2 and bax by Western blotting were detected.Results Tangeretin could effectively inhibit the proliferation of MMQ in a dose-dependent and time-dependent manner.At the same time,tangeretin induced apoptosis and down-regulated the level of bcl-2 and up-regulated the level of bax in MMQ cells.Conclusion Tangeretin maybe induced apoptosis and inhibited the proliferation of MMQ through bcl-2/bax signaling pathway.