Bcl-2、Bax、Ki67在原发性翼状胬肉组织中的表达研究

Expressions of Bcl-2,Bax and Ki67 in t he primary pterygium

目的:观察Bcl-2、Bax、Ki67在原发性翼状胬肉和正常球结膜组织中的表达。方法:将手术切除的原发性翼状胬肉和正常球结膜组织进行固定、石蜡包埋、切片,利用免疫组织化学染色方法分析Bcl-2(B细胞淋巴瘤-2)、Bax(与Bcl-2相关的X蛋白)、Ki67(细胞核相关抗原)在其中的表达情况。结果:Bcl-2、Bax、Ki67在原发性翼状胬肉组织中的阳性表达率分别为14.73%、7.00%、12.73%,在正常球结膜组织阳性表达率分别为4.00%、6.00%、0,其中Bcl-2、Ki67在原发性翼状胬肉组织头部上皮中的表达明显高于颈部和体部。结论:Bcl-2、Ki67在原发性翼状胬肉组织中呈高表达,且在胬肉头部表达最高,在正常球结膜组织中呈低表达;Bax在原发性翼状胬肉和正常球结膜组织中表达无明显差异,提示细胞凋亡失衡和异常增殖可能是原发性翼状胬肉发生发展的机制之一。

AIM:To research the expressions of B-cell lymphoma gene 2(Bcl-2),Bcl-2 associated X-protein(Bax)and Ki67(proliferating cell marker)in the primary pterygium and normal bulbar conjunctiva,to explore the pathogenesis of pterygium.METHODS:Thirty pterygium were surgically removed and 5 normal bulbar conjunctivas were also obtained.Formalin-fixed,paraffin-wax-embedded tissues were analyzed by immunohistochemistry with Bcl-2,Bax,Ki67.RESULTS:The mean positive expression of Bcl-2,Bax and Ki67 in the primary pterygium was 14.73%,7.00%and 12.73%,respectively 4.00%,6.00%and 0 in normal conjunctiva group.The expression of Bcl-2 and Ki67 in the head of primary pterygium was higher than that of neck and body.CONCLUSION:Bcl-2 and Ki67 have highly expressions in the primary pterygium,the expressions in the head of primary pterygium is the highest,the expressions in normal bulbar conjunctiva is low or no.There is no significant difference in the expression of Bax in the primary pterygium and normal bulbar conjunctiva.Apoptosis imbalance and abnormal proliferation of cells may be one of the mechanisms for the occurrence and development of the primary pterygium.