黄柏酮对大鼠自体移植静脉桥术后内膜增生的抑制作用及其机制

Inhibitory effects and its mechanism of obacunone on intimal hyperplasia after autogenous vein grafts in rats

目的探讨黄柏酮对大鼠自体移植静脉桥术后内膜增生的抑制作用及其可能作用机制。方法选取42只雄性SD大鼠,构建自体静脉移植模型后将其随机分为实验组和对照组,每组21只,实验组给予黄柏酮15 mg/(kg·d)腹腔注射,对照组注射等量生理盐水,在术后0、2、4周分别取移植术后的大鼠静脉桥,观察大鼠静脉桥组织形态,测量新生内膜厚度,检测增殖细胞核抗原(PCNA)表达水平和蛋白激酶B(Akt)磷酸化水平。结果术后2周和4周时,对照组平滑肌细胞大量增殖,内皮细胞排列欠规则,实验组移植静脉桥平滑肌细胞增殖少于对照组;实验组静脉桥新生内膜厚度薄于对照组(P<0.05),PCNA蛋白表达水平和Akt磷酸化水平低于对照组(P<0.05)。结论黄柏酮可能通过降低Akt磷酸化水平来抑制平滑肌细胞增殖,从而减轻静脉桥内膜增生。

Objective To investigate the inhibitory effects and its potential mechanism of obacunone on intimal hyperplasia after autogenous vein graft in rats.Methods Forty-two male SD rats were randomly divided into experimental group and control group after establishing the model of autologous vein grafts,with 21 rats in each group.The experimental group was treated with obacunone[15 mg/(kg·d)]by intraperitoneal injection,and the control group was injected with an equivalent volume of normal saline.The vein grafts were taken from the rats in the postoperative 0,2rd and 4th week,respectively,to observe the histomorphology,and the neointimal thickness was measured.The expression level of proliferating cell nuclear antigen(PCNA)and the phosphorylation level of protein kinase B(Akt)were detected.Results After 2 and 4 weeks of operation,massive vascular smooth muscle cell proliferation and irregular endothelial cell arrangement were observed in the control group,and the proliferation of vascular smooth muscle cells in the experimental group was less than that in the control group;the neointimal thickness of vein grafts in the experimental group was thinner than that in the control group(P<0.05),and the expression level of PCNA protein and the phosphorylation level of Akt in the experimental group were lower than those in the control group(P<0.05).Conclusion Obacunone might inhibit the proliferation of vascular smooth muscle cells by reducing the phosphorylation level of Akt,thus alleviating the intimal hyperplasia of vein grafts.