维生素C对内皮细胞和平滑肌细胞增殖的影响

Effects of vitamin C on proliferation of endothelial cells and smooth muscle cells

药物洗脱支架(Drug-eluting stent,DES)释放抗增殖的药物来抑制平滑肌细胞(Smooth muscle cells,SMCs)增殖,阻止内膜增生。但这些药物不可避免地会延迟内皮化,因此有必要寻找合适的药物选择性的抑制SMC增殖,同时促进内皮细胞(Endothelial cells,ECs)增殖。维生素C(L-ascorbic acid,L-AA)是一种用于口服或是直接加入细胞培养的药物,因此实验中在培养过夜的EC和SMC细胞中加入浓度为300μg/mL的L-AA、浓度为100μg/mL的雷帕霉素(Sirolimus,SIR)、高糖培养基(Dulbeccos modified eagle medium,DMEM)、杜氏磷酸缓冲液(Dulbeccos phosphate buffered saline,DPBS)和乙醇各100μL,培养7d观察,结果表明:SIR能够抑制EC增殖,而L-AA能够显著促进EC的增殖,同时也能够抑制SMC的生长;在培养过夜的EC和SMC细胞中加入100μL不同浓度的L-AA(1、100、300、500μg/mL和1000μg/mL),培养7d后,1、100μg/mL和300μg/mL的L-AA有利于EC增殖,而500μg/mL和1000μg/mL的L-AA会抑制EC增殖,并且300μg/mL和500μg/mL的L-AA可以显著抑制SMC增殖。由此表明维生素C可以成为潜在的用于药物洗脱支架的药物。

Drug-eluting stents(DES)release anti-proliferation drugs to inhibit the proliferation of smooth muscle cells(SMCs)and prevent intimal hyperplasia.However,these drugs inevitably delay endothelialization.Therefore,it is necessary to find the suitable drugs which selectively inhibit the proliferation of SMC and promote the proliferation of endothelial cells(ECs).Vitamin C(L-ascorbic acid[L-AA])is a kind of drug for oral administration or directly added into cell culture.Therefore,in this experiment,EC and SMC cells cultured overnight were added to 100μL L-AA(300μg/mL),100μL Sirolimus(SIR)(100μg/mL),100μL dulbecco s modified eagle medium(DMEM),100μL dulbecco s phosphate buffered saline(DPBS)and 100μL ethanol cultured for 7 days.It was found that SIR could inhibit the proliferation of EC,while L-AA could significantly promote the proliferation of EC and also inhibit the growth of SMC.After adding different concentrations of 100μL of L-AA(1,100,300,500μg/mL and 1000μg/mL)in overnight cultured EC and SMC cells for 7 days.The L-AA dosage study demonstrated that L-AA with the concentrations of 1μg/mL,100μg/mL and 300μg/mL could encourage EC proliferation,while L-AA with the concentrations of 500μg/mL and 1000μg/mL could inhibit EC proliferation.At the same time,L-AA with the concentrations of 300μg/mL and 500μg/mL could significantly inhibit SMC proliferation.Thus,this study demonstrates that vitamin C can be a potential drug for drug-eluting stents.