实时直接分析-质谱法快速检测饮料和尿液中的γ-羟基丁酸

Rapid Determination of γ-Hydroxybutyric Acid in Beverage and Urine by Direct Analysis in Real-time Mass Spectrometry

建立了一种实时直接分析-质谱法(DART-MS)用于饮料(水、碳酸饮料、啤酒)和尿液中γ-羟基丁酸(GHB)快速检测。样品经甲醇-水(1∶1,V/V)溶液稀释后,在负离子模式下,以选择离子扫描(SIR)模式进行直接定量分析。离子化气体温度为350℃,进样速率为0.5 mm/s。针对水样、碳酸饮料、啤酒、尿液样品,本方法的检出限(S/N=3)为1~2μg/m L,定量限(S/N=10)为3~5μg/m L。标准曲线线性相关系数为0.9899~0.9980,加标回收率为80.8%~115.2%,相对标准偏差为1.9%~12.8%。本方法具有样品前处理简单、分析速度快、成本低等优点,有望在大批量饮料和尿液样品的快速筛查分析中发挥作用。

A method for rapid determination ofγ-hydroxybutyric acid(GHB)in beverages(water,sodas,beer)and urine was established by direct analysis real-time mass spectrometry(DART-MS).Samples were analyzed directly after dilution with mixture of methanol and water(1∶1,V/V).Instrument parameter settings were optimized to obtain the sensitive and accurate determination of GHB.At the sample introduction speed of 0.5 mm/s,high intensity of[M-H]0x0E?Symbolm@@0x0F ions for GHB were observed in the negative ion and selection ion monitoring mode by utilization of high purity helium gas at 350℃.For different samples of water,sodas,beer and urine,the limits of detection(LODs)(S/N=3)were in the range of 1-2μg/mL,while the limits of quantification(LOQs)(S/N=10)were in the range of 3-5μg/mL.The linear correlation coefficients of the standard curves with different sample matrixes were between 0.9899 and 0.9980.The recoveries were in the range of 80.8%-115.2%with the relative standard deviations of 1.9%-12.8%.With its rapid analysis and simple pretreatment steps,the method is expected to have a strong advantage in the rapid screening analysis of large quantities of beverage and urine.