摘要
为了解三穗鸭JAK2基因特征,采用分段PCR方法获取三穗麻鸭JAK2基因并进行克隆与测序,应用生物信息学相关软件进行基因分子特征分析。结果:三穗麻鸭JAK2基因全长3 393 bp,可编码1 131个氨基酸;与原鸡的核苷酸序列同源性达94.5%,氨基酸同源性达97.6%;进化树分析显示,该基因与鸡属动物亲缘关系最近。
To analyze the molecular characteristics of Sansui duck JAK 2 gene and its encoding protein,the JAK 2 gene of Sansui duck was obtained by the PCR method,then cloned and sequenced,and the molecular characterization of this gene was analyzed by bioinformatics software.The results showed that the length of JAK 2 gene of Sansui duck was 3 393 bp,which encode 1 131 amino acids.The homology of this nucleotide sequences reached 97.6%with that of anser,and the homology of amino acid was 94.5%.The gene was closely related to the chicken genus by phylogenetic tree analysis.
作者
张美兰
张宇
邹作成
田芳
张飘
龙立书
华敏
程振涛
周碧君
文明
Zhang Meilan;Zhang Yu;Zou Zuocheng;Tian Fang;Zhang Piao;Long Lishu;Hua Min;Cheng Zhentao;Zhou Bijun;Wen Ming(College of Animal Science,Guizhou University,Guiyang Guizhou 550025,China;Key Laboratory of Animal Diseases and Veterinary Public Health in Guizhou Province,Guiyang Guizhou 550025,China)
出处
《贵州畜牧兽医》
2018年第2期6-9,共4页
Guizhou Journal of Animal Husbandry & Veterinary Medicine
基金
贵州大学SRT计划项目"鸭免疫相关基因(NLRC)克隆与分析"(贵大SRT字[2016]180号)
国家自然科学基金项目(项目批准号31560703)
贵州省科技创新人才团队建设项目"贵州省动物疫病防控与兽医公共卫生保障科技创新人才团队"(黔科合人才团队[2015]4016号)
