玉米叶高点长等叶型性状QTL定位及上位性效应分析

QTL mapping and epistatic effect analysis of high point long and other leaf shape related traits in maize

以由综3×豫87-1衍生的一套包含223个家系的RIL群体为材料,通过3个地点的表型鉴定,借助由1 243个SNP标记构建的遗传连锁图谱对玉米叶高点长、叶长、叶宽和叶面积进行QTL定位及上位性效应分析。结果表明,4个叶型性状共检测到10个显著的QTL,这些QTL与环境的互作均未达到显著水平。在这些QTL中,调控叶高点长的q Lf5-1,q Lf8-1和调控叶长的q LL8-1,分别解释表型变异的7.20%,6.06%和6.39%,说明这3个主效QTL是调控叶型性状的重要位点。上位性效应分析共检测到6对位点间互作,互作效应为2.30%~7.39%,属于非显著QTL位点对互作,其中2对位点互作的上位性效应与环境互作效应显著,说明上位性互作效应在叶型性状的遗传中占有一定的比例,同时也受环境的影响。

Using a set of RIL populations containing 223 pedigrees derived from Zong 3×Yu 87-1 as material,the phenotypes of the three sites were used to identify the height of the maize leaves using genetic linkage maps constructed from 1 243 SNP markers for leaf length,leaf width and leaf area for QTL mapping and epistasis analysis.The results showed that 10 significant QTLs were detected in 4 leaf-type traits.The interactions between these QTLs and the environment did not reach significant levels.In these QTLs,qLf5-1,qLf8-1,and qLL8-1,which regulate long leaf height,accounted respectively for 7.20%,6.06%,and 6.39%of phenotypic variation,indicating that these three major QTLs are regulating the important locus of leaf-shaped traits.By epistasis effect analysis,a total of 6 pairs of sites interactions were detected,and the interaction effect was 2.30%~7.39%,which belonged to non-significant QTL site interaction,in which the epistasis effect and environment interaction of the two pairs of sites interacted with each other.The effect was significant,indicating that epistatic effects accounted for a certain proportion of the inheritance of leaf-type traits,but also affected by the environment.