陆地棉半矮秆突变体sd株高对外源激素的敏感性

Research on Exogenous Hormones Sensitivity on Plant Height of a Semi-Dwarf Mutant in Upland Cotton

【目的】研究外源激素对棉花矮化突变体株高的影响,确定其对激素的敏感性,为利用其分析棉花株高遗传基础与激素调控关系提供依据。【方法】以棉花品种新陆早17号(CK)及其矮化突变体sd为材料,采用GA3、IAA和BR激素分别处理后,研究不同激素和不同浓度处理对棉花株高的影响。【结果】突变体sd的株高约为新陆早17号的四分之三,突变体sd是一份典型的陆地棉半矮秆突变体。不同浓度GA_3处理后,新陆早17号的株高变化量高于突变体,50 mg/L的GA_3处理能使突变体sd的株高恢复到新陆早17号的株高水平,激素影响棉花株高的主次顺序为GA_3>BR>IAA,株高与GA_3具有显著的正相关性。【结论】突变体sd是一份典型的陆地棉半矮秆突变体,突变体sd的株高对GA_3敏感。

【Objective】The aim of this study is to illuminate the sensibility of the exogenous hormones to plant height of a dwarf mutant in upland cotton so as to determine which kind of hormone sensitivity the mutant belongs to,which will facilitate dissecting the relationship between the genetic basis of plant height and hormonal regulation in cotton.【Method】The cultivated upland cotton variety Xinluzao 17 and its dwarf mutant were used as experimental materials.The plant height variations of the two cottons were observed under different hormone types and different concentrations.【Result】The mutant was approximately three-quarters of the plant height of Xinluzao17,which indicated that the mutant was a typical semi-dwarf mutant in upland cotton.After the cottons were treated with GA 3,the plant height of Xinluzao17 was higher than that of the mutant.Treated with 50 mg/L GA3,plant height of the mutant could be resumed to the plant height of Xinluzao17,a normal plant height cultivar.It suggested that the plant height of the mutant was sensitive to GA 3.The order of the effects of phytohormone on cotton plant height was GA 3>BR>IAA.Pearson correlation analysis showed that the plant height had a significant positive correlation with GA 3.It further suggested that the mutant was a GA 3 sensitive dwarf.【Conclusion】The mutant is a typical semi-dwarf in upland cotton.The plant height of the mutant is sensitive to exogenous GA 3.